Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25265 and BWI76_RS25270 are separated by 22 nucleotides BWI76_RS25270 and BWI76_RS25275 are separated by 65 nucleotides BWI76_RS25275 and BWI76_RS25280 are separated by 45 nucleotides
BWI76_RS25265: BWI76_RS25265 - RNA polymerase factor sigma-54, at 5,251,847 to 5,253,280
_RS25265
BWI76_RS25270: BWI76_RS25270 - ribosome hibernation promoting factor, at 5,253,303 to 5,253,590
_RS25270
BWI76_RS25275: BWI76_RS25275 - PTS IIA-like nitrogen regulatory protein PtsN, at 5,253,656 to 5,254,144
_RS25275
BWI76_RS25280: BWI76_RS25280 - RNase adaptor protein RapZ, at 5,254,190 to 5,255,044
_RS25280
Position (kb) 5253
5254 Strain fitness (log2 ratio) -1
0
1 at 5253.303 kb on + strand at 5253.477 kb on + strand, within BWI76_RS25270 at 5253.483 kb on + strand, within BWI76_RS25270 at 5253.502 kb on + strand, within BWI76_RS25270 at 5254.184 kb on + strand at 5254.201 kb on - strand at 5254.555 kb on + strand, within BWI76_RS25280
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 5,253,303 + +0.1 5,253,477 + BWI76_RS25270 0.60 -0.1 5,253,483 + BWI76_RS25270 0.62 -1.3 5,253,502 + BWI76_RS25270 0.69 -1.5 5,254,184 + -0.0 5,254,201 - -1.3 5,254,555 + BWI76_RS25280 0.43 +0.5
Or see this region's nucleotide sequence