Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21540 and BWI76_RS21545 are separated by 65 nucleotides BWI76_RS21545 and BWI76_RS21550 overlap by 36 nucleotides BWI76_RS21550 and BWI76_RS21555 are separated by 13 nucleotides BWI76_RS21555 and BWI76_RS21560 are separated by 86 nucleotides
BWI76_RS21540: BWI76_RS21540 - membrane protein, at 4,510,017 to 4,511,306
_RS21540
BWI76_RS21545: BWI76_RS21545 - nucleotide exchange factor GrpE, at 4,511,372 to 4,511,962
_RS21545
BWI76_RS21550: BWI76_RS21550 - inorganic polyphosphate kinase, at 4,511,927 to 4,512,073
_RS21550
BWI76_RS21555: BWI76_RS21555 - NAD(+) kinase, at 4,512,087 to 4,512,965
_RS21555
BWI76_RS21560: BWI76_RS21560 - DNA repair protein RecN, at 4,513,052 to 4,514,713
_RS21560
Position (kb) 4512
4513 Strain fitness (log2 ratio) -2
-1
0
1 at 4511.210 kb on + strand at 4511.218 kb on - strand at 4511.232 kb on + strand at 4513.141 kb on + strand at 4513.141 kb on + strand at 4513.823 kb on + strand, within BWI76_RS21560 at 4513.838 kb on - strand, within BWI76_RS21560
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,511,210 + -1.8 4,511,218 - -0.3 4,511,232 + -1.5 4,513,141 + -0.1 4,513,141 + +0.4 4,513,823 + BWI76_RS21560 0.46 +0.1 4,513,838 - BWI76_RS21560 0.47 -0.6
Or see this region's nucleotide sequence