Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18815

Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18810 and BWI76_RS18815 are separated by 16 nucleotidesBWI76_RS18815 and BWI76_RS18820 are separated by 55 nucleotides BWI76_RS18810: BWI76_RS18810 - nitrogenase iron protein, at 3,901,391 to 3,902,272 _RS18810 BWI76_RS18815: BWI76_RS18815 - nitrogenase molybdenum-iron protein alpha chain, at 3,902,289 to 3,903,737 _RS18815 BWI76_RS18820: BWI76_RS18820 - nitrogenase molybdenum-iron protein subunit beta, at 3,903,793 to 3,905,355 _RS18820 Position (kb) 3902 3903 3904Strain fitness (log2 ratio) -1 0 1 2at 3901.401 kb on - strandat 3901.456 kb on - strandat 3901.456 kb on - strandat 3901.791 kb on - strand, within BWI76_RS18810at 3902.172 kb on - strand, within BWI76_RS18810at 3902.334 kb on + strandat 3902.450 kb on - strand, within BWI76_RS18815at 3903.425 kb on - strand, within BWI76_RS18815at 3903.471 kb on - strand, within BWI76_RS18815at 3903.622 kb on + strandat 3903.641 kb on - strandat 3903.689 kb on - strandat 3903.864 kb on - strandat 3904.061 kb on + strand, within BWI76_RS18820at 3904.061 kb on + strand, within BWI76_RS18820at 3904.069 kb on - strand, within BWI76_RS18820

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,901,401 - +1.8
3,901,456 - +1.2
3,901,456 - +0.9
3,901,791 - BWI76_RS18810 0.45 +0.5
3,902,172 - BWI76_RS18810 0.89 +0.5
3,902,334 + -0.6
3,902,450 - BWI76_RS18815 0.11 -1.0
3,903,425 - BWI76_RS18815 0.78 -1.6
3,903,471 - BWI76_RS18815 0.82 -0.3
3,903,622 + +0.4
3,903,641 - +0.5
3,903,689 - +0.1
3,903,864 - +0.6
3,904,061 + BWI76_RS18820 0.17 -0.3
3,904,061 + BWI76_RS18820 0.17 -0.5
3,904,069 - BWI76_RS18820 0.18 +0.1

Or see this region's nucleotide sequence