Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15450

Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
3,236,410	+				+1.5
3,236,567	-		BWI76_RS15440	0.67	+0.7
3,236,581	+		BWI76_RS15440	0.72	+0.9
3,236,776	-				+1.8
3,236,863	+				+0.7
3,236,869	+				+0.9
3,236,892	+				+0.1
3,236,899	+				+1.2
3,236,904	+				+0.9
3,236,907	-				+0.7
3,236,924	-				-0.2
3,236,953	+				+0.4
3,236,961	-				-2.2
3,236,978	-				-2.4
3,237,015	+				-0.3
3,237,015	+				+0.5
3,237,015	+				+0.7
3,237,015	+				+0.8
3,237,165	-		BWI76_RS15445	0.49	-1.0
3,237,170	+		BWI76_RS15445	0.51	+0.4
3,237,198	-		BWI76_RS15445	0.61	+0.5
3,237,212	-		BWI76_RS15445	0.66	+0.4
3,237,228	+		BWI76_RS15445	0.72	+1.1
3,237,250	+		BWI76_RS15445	0.80	+1.8
3,237,356	-				-2.8
3,237,360	+				-0.1
3,237,368	-				+0.4
3,237,368	-				-0.0
3,237,389	+				+0.1
3,237,448	+		BWI76_RS15450	0.26	-1.0
3,237,456	-		BWI76_RS15450	0.29	+0.2
3,237,456	-		BWI76_RS15450	0.29	-1.4
3,237,610	-		BWI76_RS15450	0.78	-1.7
3,237,781	+				-2.8
3,237,781	+				+0.7
3,237,801	+				+0.3
3,237,809	-				+0.1
3,237,855	+				-0.1
3,237,863	-				+1.1
3,237,911	+		BWI76_RS15455	0.13	+0.1
3,237,919	-		BWI76_RS15455	0.14	-0.4
3,237,919	-		BWI76_RS15455	0.14	-0.9
3,237,919	-		BWI76_RS15455	0.14	-1.1
3,237,919	-		BWI76_RS15455	0.14	-2.1
3,237,946	-		BWI76_RS15455	0.19	+0.3
3,237,947	+		BWI76_RS15455	0.19	+2.5
3,238,001	+		BWI76_RS15455	0.29	-0.3
3,238,045	+		BWI76_RS15455	0.38	-0.6
3,238,053	-		BWI76_RS15455	0.39	+0.3
3,238,104	+		BWI76_RS15455	0.49	+0.2
3,238,154	+		BWI76_RS15455	0.58	+0.7
3,238,172	+		BWI76_RS15455	0.61	-1.3
3,238,172	+		BWI76_RS15455	0.61	-1.3
3,238,185	-		BWI76_RS15455	0.64	+1.1
3,238,295	+				-1.5
3,238,295	+		BWI76_RS15455	0.84	+0.5
3,238,295	+		BWI76_RS15455	0.84	-2.5
3,238,295	+		BWI76_RS15455	0.84	+0.3
3,238,295	+		BWI76_RS15455	0.84	-0.1
3,238,295	+		BWI76_RS15455	0.84	-0.1
3,238,295	+		BWI76_RS15455	0.84	-1.5
3,238,295	+				-1.3
3,238,295	+		BWI76_RS15455	0.84	-2.7
3,238,295	+		BWI76_RS15455	0.84	+0.1
3,238,303	-		BWI76_RS15455	0.85	-1.9
3,238,303	-		BWI76_RS15455	0.85	+0.6
3,238,314	+		BWI76_RS15455	0.87	-1.7
3,238,327	+		BWI76_RS15455	0.90	+2.4
3,238,383	-				-0.7
3,238,385	+				-0.3
3,238,393	-				+1.4
3,238,395	+				-1.7
3,238,398	-				-1.5
3,238,403	-				+1.2
3,238,421	-				+1.3
3,238,421	-				-0.3
3,238,428	+				-0.9
3,238,436	+				+1.0
3,238,444	-				-0.9
3,238,444	-				-1.3
3,238,481	-				-0.6
3,238,501	-				-0.1
3,238,507	+				-3.1
3,238,534	-				-0.1
3,238,596	+		BWI76_RS15460	0.21	-2.9
3,238,596	+		BWI76_RS15460	0.21	-1.4
3,238,596	+		BWI76_RS15460	0.21	-1.8
3,238,596	+		BWI76_RS15460	0.21	+1.5
3,238,596	+		BWI76_RS15460	0.21	-0.4
3,238,596	+		BWI76_RS15460	0.21	-1.6
3,238,604	-		BWI76_RS15460	0.23	+0.5
3,238,604	-		BWI76_RS15460	0.23	-3.7
3,238,606	+		BWI76_RS15460	0.23	-0.1