Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12500

Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12490 and BWI76_RS12495 are separated by 41 nucleotidesBWI76_RS12495 and BWI76_RS12500 are separated by 101 nucleotidesBWI76_RS12500 and BWI76_RS12505 overlap by 10 nucleotidesBWI76_RS12505 and BWI76_RS12510 are separated by 188 nucleotides BWI76_RS12490: BWI76_RS12490 - AbrB family transcriptional regulator, at 2,609,021 to 2,610,076 _RS12490 BWI76_RS12495: BWI76_RS12495 - 2-Cys peroxiredoxin, at 2,610,118 to 2,610,627 _RS12495 BWI76_RS12500: BWI76_RS12500 - dipeptide epimerase, at 2,610,729 to 2,611,694 _RS12500 BWI76_RS12505: BWI76_RS12505 - murein peptide amidase A, at 2,611,685 to 2,612,398 _RS12505 BWI76_RS12510: BWI76_RS12510 - peptide ABC transporter substrate-binding protein, at 2,612,587 to 2,614,203 _RS12510 Position (kb) 2610 2611 2612Strain fitness (log2 ratio) -2 -1 0 1at 2609.745 kb on - strand, within BWI76_RS12490at 2609.988 kb on - strandat 2610.154 kb on + strandat 2610.527 kb on + strand, within BWI76_RS12495at 2611.041 kb on - strand, within BWI76_RS12500at 2611.055 kb on + strand, within BWI76_RS12500at 2611.079 kb on + strand, within BWI76_RS12500at 2611.497 kb on + strand, within BWI76_RS12500at 2611.695 kb on - strandat 2612.394 kb on + strandat 2612.401 kb on - strandat 2612.484 kb on + strandat 2612.484 kb on + strandat 2612.592 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,609,745 - BWI76_RS12490 0.69 +0.6
2,609,988 - -0.4
2,610,154 + +1.1
2,610,527 + BWI76_RS12495 0.80 +0.6
2,611,041 - BWI76_RS12500 0.32 +0.6
2,611,055 + BWI76_RS12500 0.34 +1.4
2,611,079 + BWI76_RS12500 0.36 +1.5
2,611,497 + BWI76_RS12500 0.80 +0.7
2,611,695 - -0.6
2,612,394 + -1.8
2,612,401 - +0.8
2,612,484 + -1.3
2,612,484 + +0.6
2,612,592 - -0.8

Or see this region's nucleotide sequence