Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11160

Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11150 and BWI76_RS11155 are separated by 12 nucleotidesBWI76_RS11155 and BWI76_RS11160 overlap by 20 nucleotidesBWI76_RS11160 and BWI76_RS11165 are separated by 19 nucleotides BWI76_RS11150: BWI76_RS11150 - membrane protein, at 2,350,996 to 2,351,370 _RS11150 BWI76_RS11155: BWI76_RS11155 - penicillin-binding protein activator LpoB, at 2,351,383 to 2,352,018 _RS11155 BWI76_RS11160: BWI76_RS11160 - thiamine kinase, at 2,351,999 to 2,352,817 _RS11160 BWI76_RS11165: BWI76_RS11165 - beta-N-acetylhexosaminidase, at 2,352,837 to 2,353,859 _RS11165 Position (kb) 2351 2352 2353Strain fitness (log2 ratio) -1 0 1at 2351.192 kb on - strand, within BWI76_RS11150at 2351.319 kb on - strand, within BWI76_RS11150at 2351.400 kb on - strandat 2351.658 kb on - strand, within BWI76_RS11155at 2351.881 kb on - strand, within BWI76_RS11155at 2352.007 kb on - strandat 2352.066 kb on - strandat 2352.951 kb on - strandat 2353.075 kb on - strand, within BWI76_RS11165at 2353.102 kb on + strand, within BWI76_RS11165at 2353.102 kb on + strand, within BWI76_RS11165at 2353.110 kb on - strand, within BWI76_RS11165at 2353.206 kb on + strand, within BWI76_RS11165at 2353.214 kb on - strand, within BWI76_RS11165at 2353.799 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,351,192 - BWI76_RS11150 0.52 -1.6
2,351,319 - BWI76_RS11150 0.86 +1.0
2,351,400 - -1.2
2,351,658 - BWI76_RS11155 0.43 -0.3
2,351,881 - BWI76_RS11155 0.78 +0.5
2,352,007 - +0.7
2,352,066 - +0.7
2,352,951 - -1.1
2,353,075 - BWI76_RS11165 0.23 -0.1
2,353,102 + BWI76_RS11165 0.26 +0.3
2,353,102 + BWI76_RS11165 0.26 -0.2
2,353,110 - BWI76_RS11165 0.27 -1.1
2,353,206 + BWI76_RS11165 0.36 -0.4
2,353,214 - BWI76_RS11165 0.37 +1.5
2,353,799 - -0.7

Or see this region's nucleotide sequence