Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11010 and BWI76_RS11015 are separated by 9 nucleotides BWI76_RS11015 and BWI76_RS11020 are separated by 2 nucleotides BWI76_RS11020 and BWI76_RS11025 are separated by 112 nucleotides
BWI76_RS11010: BWI76_RS11010 - 30S ribosomal protein S5 alanine N-acetyltransferase, at 2,322,346 to 2,322,930
_RS11010
BWI76_RS11015: BWI76_RS11015 - hypothetical protein, at 2,322,940 to 2,323,587
_RS11015
BWI76_RS11020: BWI76_RS11020 - oxidoreductase family, NAD-binding protein, at 2,323,590 to 2,324,513
_RS11020
BWI76_RS11025: BWI76_RS11025 - lipid II flippase MurJ, at 2,324,626 to 2,326,161
_RS11025
Position (kb)
2323
2324
2325 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2322.708 kb on - strand, within BWI76_RS11010 at 2322.751 kb on + strand, within BWI76_RS11010 at 2323.022 kb on - strand, within BWI76_RS11015 at 2323.042 kb on - strand, within BWI76_RS11015 at 2323.186 kb on - strand, within BWI76_RS11015 at 2323.889 kb on + strand, within BWI76_RS11020 at 2324.161 kb on - strand, within BWI76_RS11020 at 2324.368 kb on + strand, within BWI76_RS11020 at 2324.376 kb on - strand, within BWI76_RS11020 at 2324.376 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,322,708 - BWI76_RS11010 0.62 -0.3 2,322,751 + BWI76_RS11010 0.69 +0.1 2,323,022 - BWI76_RS11015 0.13 +0.9 2,323,042 - BWI76_RS11015 0.16 -2.8 2,323,186 - BWI76_RS11015 0.38 -0.2 2,323,889 + BWI76_RS11020 0.32 -1.9 2,324,161 - BWI76_RS11020 0.62 +0.2 2,324,368 + BWI76_RS11020 0.84 -0.7 2,324,376 - BWI76_RS11020 0.85 +0.1 2,324,376 - +0.5
Or see this region's nucleotide sequence