Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09600

Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09595 and BWI76_RS09600 overlap by 4 nucleotidesBWI76_RS09600 and BWI76_RS09605 are separated by 68 nucleotidesBWI76_RS09605 and BWI76_RS09610 are separated by 324 nucleotidesBWI76_RS09610 and BWI76_RS09615 are separated by 30 nucleotides BWI76_RS09595: BWI76_RS09595 - macrolide transporter subunit MacA, at 2,007,481 to 2,008,596 _RS09595 BWI76_RS09600: BWI76_RS09600 - macrolide ABC transporter permease/ATP-binding protein MacB, at 2,008,593 to 2,010,539 _RS09600 BWI76_RS09605: BWI76_RS09605 - cold-shock protein, at 2,010,608 to 2,010,838 _RS09605 BWI76_RS09610: BWI76_RS09610 - ATP-dependent Clp protease adaptor ClpS, at 2,011,163 to 2,011,480 _RS09610 BWI76_RS09615: BWI76_RS09615 - ATP-dependent Clp protease ATP-binding subunit ClpA, at 2,011,511 to 2,013,793 _RS09615 Position (kb) 2008 2009 2010 2011Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2007.616 kb on + strand, within BWI76_RS09595at 2007.915 kb on - strand, within BWI76_RS09595at 2008.219 kb on + strand, within BWI76_RS09595at 2008.422 kb on + strand, within BWI76_RS09595at 2008.669 kb on - strandat 2008.822 kb on + strand, within BWI76_RS09600at 2009.085 kb on - strand, within BWI76_RS09600at 2009.174 kb on - strand, within BWI76_RS09600at 2009.179 kb on - strand, within BWI76_RS09600at 2009.291 kb on - strand, within BWI76_RS09600at 2009.342 kb on - strand, within BWI76_RS09600at 2009.839 kb on + strand, within BWI76_RS09600at 2009.990 kb on + strand, within BWI76_RS09600at 2010.135 kb on + strand, within BWI76_RS09600at 2010.151 kb on + strand, within BWI76_RS09600at 2010.314 kb on - strand, within BWI76_RS09600at 2010.584 kb on + strandat 2010.592 kb on - strandat 2010.832 kb on + strandat 2011.146 kb on - strandat 2011.165 kb on + strandat 2011.267 kb on - strand, within BWI76_RS09610at 2011.337 kb on - strand, within BWI76_RS09610at 2011.456 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,007,616 + BWI76_RS09595 0.12 +0.4
2,007,915 - BWI76_RS09595 0.39 +0.5
2,008,219 + BWI76_RS09595 0.66 -0.1
2,008,422 + BWI76_RS09595 0.84 -3.8
2,008,669 - +0.4
2,008,822 + BWI76_RS09600 0.12 +0.1
2,009,085 - BWI76_RS09600 0.25 +0.3
2,009,174 - BWI76_RS09600 0.30 -0.3
2,009,179 - BWI76_RS09600 0.30 -0.6
2,009,291 - BWI76_RS09600 0.36 -0.4
2,009,342 - BWI76_RS09600 0.38 -0.1
2,009,839 + BWI76_RS09600 0.64 +0.5
2,009,990 + BWI76_RS09600 0.72 -1.0
2,010,135 + BWI76_RS09600 0.79 -0.0
2,010,151 + BWI76_RS09600 0.80 -0.4
2,010,314 - BWI76_RS09600 0.88 -1.1
2,010,584 + -0.9
2,010,592 - +0.4
2,010,832 + +0.4
2,011,146 - -0.7
2,011,165 + +0.6
2,011,267 - BWI76_RS09610 0.33 +0.5
2,011,337 - BWI76_RS09610 0.55 -0.2
2,011,456 + -0.2

Or see this region's nucleotide sequence