Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09540 and BWI76_RS09545 are separated by 96 nucleotides BWI76_RS09545 and BWI76_RS09550 are separated by 10 nucleotides
BWI76_RS09540: BWI76_RS09540 - hypothetical protein, at 1,994,714 to 1,995,727
_RS09540
BWI76_RS09545: BWI76_RS09545 - NAD-dependent epimerase/dehydratase, at 1,995,824 to 1,997,257
_RS09545
BWI76_RS09550: BWI76_RS09550 - low-specificity L-threonine aldolase, at 1,997,268 to 1,998,269
_RS09550
Position (kb)
1995
1996
1997
1998 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1994.934 kb on + strand, within BWI76_RS09540 at 1995.367 kb on + strand, within BWI76_RS09540 at 1995.592 kb on - strand, within BWI76_RS09540 at 1995.999 kb on - strand, within BWI76_RS09545 at 1995.999 kb on - strand, within BWI76_RS09545 at 1996.322 kb on - strand, within BWI76_RS09545 at 1996.669 kb on - strand, within BWI76_RS09545 at 1996.874 kb on + strand, within BWI76_RS09545 at 1997.060 kb on - strand, within BWI76_RS09545 at 1997.075 kb on + strand, within BWI76_RS09545 at 1997.092 kb on + strand, within BWI76_RS09545 at 1997.274 kb on - strand at 1997.370 kb on - strand, within BWI76_RS09550 at 1997.375 kb on - strand, within BWI76_RS09550 at 1997.556 kb on - strand, within BWI76_RS09550 at 1997.744 kb on - strand, within BWI76_RS09550 at 1997.750 kb on - strand, within BWI76_RS09550 at 1998.198 kb on - strand at 1998.235 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,994,934 + BWI76_RS09540 0.22 -0.1 1,995,367 + BWI76_RS09540 0.64 -0.8 1,995,592 - BWI76_RS09540 0.87 +0.4 1,995,999 - BWI76_RS09545 0.12 +0.2 1,995,999 - BWI76_RS09545 0.12 +3.4 1,996,322 - BWI76_RS09545 0.35 -1.0 1,996,669 - BWI76_RS09545 0.59 -0.1 1,996,874 + BWI76_RS09545 0.73 -0.1 1,997,060 - BWI76_RS09545 0.86 +1.1 1,997,075 + BWI76_RS09545 0.87 +1.6 1,997,092 + BWI76_RS09545 0.88 +1.2 1,997,274 - +1.0 1,997,370 - BWI76_RS09550 0.10 +2.3 1,997,375 - BWI76_RS09550 0.11 -0.3 1,997,556 - BWI76_RS09550 0.29 +0.3 1,997,744 - BWI76_RS09550 0.48 -0.2 1,997,750 - BWI76_RS09550 0.48 +0.7 1,998,198 - -0.5 1,998,235 + -0.2
Or see this region's nucleotide sequence