Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08295

Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08290 and BWI76_RS08295 are separated by 56 nucleotidesBWI76_RS08295 and BWI76_RS08300 are separated by 3 nucleotidesBWI76_RS08300 and BWI76_RS08305 overlap by 4 nucleotides BWI76_RS08290: BWI76_RS08290 - phosphoglucomutase, alpha-D-glucose phosphate-specific, at 1,753,525 to 1,755,165 _RS08290 BWI76_RS08295: BWI76_RS08295 - hypothetical protein, at 1,755,222 to 1,755,641 _RS08295 BWI76_RS08300: BWI76_RS08300 - DNA-binding response regulator, at 1,755,645 to 1,756,322 _RS08300 BWI76_RS08305: BWI76_RS08305 - two-component sensor histidine kinase, at 1,756,319 to 1,759,006 _RS08305 Position (kb) 1755 1756Strain fitness (log2 ratio) -2 -1 0 1at 1755.272 kb on + strand, within BWI76_RS08295at 1755.275 kb on - strand, within BWI76_RS08295at 1755.281 kb on + strand, within BWI76_RS08295at 1755.294 kb on - strand, within BWI76_RS08295at 1755.329 kb on - strand, within BWI76_RS08295at 1755.329 kb on - strand, within BWI76_RS08295at 1755.393 kb on - strand, within BWI76_RS08295at 1755.418 kb on - strand, within BWI76_RS08295at 1755.624 kb on - strandat 1756.151 kb on - strand, within BWI76_RS08300at 1756.369 kb on + strandat 1756.515 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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1,755,272 + BWI76_RS08295 0.12 -0.5
1,755,275 - BWI76_RS08295 0.13 +0.1
1,755,281 + BWI76_RS08295 0.14 -2.5
1,755,294 - BWI76_RS08295 0.17 +0.8
1,755,329 - BWI76_RS08295 0.25 +0.0
1,755,329 - BWI76_RS08295 0.25 -1.2
1,755,393 - BWI76_RS08295 0.41 -0.4
1,755,418 - BWI76_RS08295 0.47 -1.7
1,755,624 - -0.3
1,756,151 - BWI76_RS08300 0.75 +0.0
1,756,369 + -0.2
1,756,515 + +0.7

Or see this region's nucleotide sequence