Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08275

Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08265 and BWI76_RS08270 are separated by 291 nucleotidesBWI76_RS08270 and BWI76_RS08275 are separated by 50 nucleotidesBWI76_RS08275 and BWI76_RS08280 are separated by 133 nucleotides BWI76_RS08265: BWI76_RS08265 - transcriptional repressor, at 1,750,135 to 1,750,587 _RS08265 BWI76_RS08270: BWI76_RS08270 - flavodoxin, at 1,750,879 to 1,751,508 _RS08270 BWI76_RS08275: BWI76_RS08275 - LexA regulated protein, at 1,751,559 to 1,751,852 _RS08275 BWI76_RS08280: BWI76_RS08280 - acyl-CoA esterase, at 1,751,986 to 1,752,759 _RS08280 Position (kb) 1751 1752Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1750.688 kb on + strandat 1750.810 kb on + strandat 1751.518 kb on + strandat 1751.526 kb on - strandat 1751.571 kb on - strandat 1751.722 kb on + strand, within BWI76_RS08275at 1751.754 kb on - strand, within BWI76_RS08275at 1752.008 kb on - strandat 1752.163 kb on - strand, within BWI76_RS08280at 1752.589 kb on + strand, within BWI76_RS08280at 1752.605 kb on - strand, within BWI76_RS08280

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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1,750,688 + -0.2
1,750,810 + -0.0
1,751,518 + +0.5
1,751,526 - -0.3
1,751,571 - -3.6
1,751,722 + BWI76_RS08275 0.55 -2.2
1,751,754 - BWI76_RS08275 0.66 -1.6
1,752,008 - -0.0
1,752,163 - BWI76_RS08280 0.23 +1.1
1,752,589 + BWI76_RS08280 0.78 +2.4
1,752,605 - BWI76_RS08280 0.80 -0.1

Or see this region's nucleotide sequence