Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06285 and BWI76_RS06290 are separated by 170 nucleotides BWI76_RS06290 and BWI76_RS06295 are separated by 91 nucleotides BWI76_RS06295 and BWI76_RS06300 are separated by 53 nucleotides
BWI76_RS06285: BWI76_RS06285 - peroxiredoxin, at 1,323,566 to 1,324,168
_RS06285
BWI76_RS06290: BWI76_RS06290 - ACP phosphodiesterase, at 1,324,339 to 1,324,920
_RS06290
BWI76_RS06295: BWI76_RS06295 - S-adenosylmethionine:tRNA ribosyltransferase-isomerase, at 1,325,012 to 1,326,082
_RS06295
BWI76_RS06300: BWI76_RS06300 - tRNA guanosine(34) transglycosylase Tgt, at 1,326,136 to 1,327,263
_RS06300
Position (kb)
1325
1326
1327 Strain fitness (log2 ratio)
-2
-1
0
1 at 1324.017 kb on - strand, within BWI76_RS06285 at 1324.044 kb on + strand, within BWI76_RS06285 at 1324.237 kb on - strand at 1324.530 kb on + strand, within BWI76_RS06290 at 1325.098 kb on + strand at 1325.367 kb on + strand, within BWI76_RS06295 at 1325.389 kb on + strand, within BWI76_RS06295 at 1325.503 kb on + strand, within BWI76_RS06295 at 1325.563 kb on - strand, within BWI76_RS06295 at 1325.675 kb on - strand, within BWI76_RS06295 at 1325.827 kb on + strand, within BWI76_RS06295 at 1325.907 kb on + strand, within BWI76_RS06295 at 1326.300 kb on + strand, within BWI76_RS06300 at 1326.781 kb on + strand, within BWI76_RS06300 at 1327.013 kb on - strand, within BWI76_RS06300
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,324,017 - BWI76_RS06285 0.75 +1.6 1,324,044 + BWI76_RS06285 0.79 +0.2 1,324,237 - -2.0 1,324,530 + BWI76_RS06290 0.33 -0.3 1,325,098 + -0.2 1,325,367 + BWI76_RS06295 0.33 -1.9 1,325,389 + BWI76_RS06295 0.35 -1.3 1,325,503 + BWI76_RS06295 0.46 -1.6 1,325,563 - BWI76_RS06295 0.51 +0.5 1,325,675 - BWI76_RS06295 0.62 -0.8 1,325,827 + BWI76_RS06295 0.76 -0.7 1,325,907 + BWI76_RS06295 0.84 -2.0 1,326,300 + BWI76_RS06300 0.15 -0.1 1,326,781 + BWI76_RS06300 0.57 +0.9 1,327,013 - BWI76_RS06300 0.78 +0.9
Or see this region's nucleotide sequence