Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05555 and BWI76_RS05560 are separated by 76 nucleotides BWI76_RS05560 and BWI76_RS05565 are separated by 2 nucleotides BWI76_RS05565 and BWI76_RS05570 are separated by 2 nucleotides BWI76_RS05570 and BWI76_RS05575 overlap by 4 nucleotides
BWI76_RS05555: BWI76_RS05555 - Crl family RNA polymerase assembly factor, at 1,164,187 to 1,164,585
_RS05555
BWI76_RS05560: BWI76_RS05560 - serine acetyltransferase, at 1,164,662 to 1,165,168
_RS05560
BWI76_RS05565: BWI76_RS05565 - beta-galactosidase, at 1,165,171 to 1,166,589
_RS05565
BWI76_RS05570: BWI76_RS05570 - hypothetical protein, at 1,166,592 to 1,167,002
_RS05570
BWI76_RS05575: BWI76_RS05575 - group 1 glycosyl transferase, at 1,166,999 to 1,168,114
_RS05575
Position (kb)
1165
1166
1167 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1164.198 kb on - strand at 1164.207 kb on + strand at 1164.207 kb on + strand at 1164.215 kb on - strand at 1164.296 kb on + strand, within BWI76_RS05555 at 1164.396 kb on - strand, within BWI76_RS05555 at 1165.323 kb on - strand, within BWI76_RS05565 at 1165.330 kb on + strand, within BWI76_RS05565 at 1165.348 kb on - strand, within BWI76_RS05565 at 1165.348 kb on - strand, within BWI76_RS05565 at 1165.383 kb on + strand, within BWI76_RS05565 at 1165.614 kb on + strand, within BWI76_RS05565 at 1165.616 kb on + strand, within BWI76_RS05565 at 1165.616 kb on + strand, within BWI76_RS05565 at 1165.652 kb on + strand, within BWI76_RS05565 at 1165.659 kb on - strand, within BWI76_RS05565 at 1165.670 kb on + strand, within BWI76_RS05565 at 1165.678 kb on - strand, within BWI76_RS05565 at 1165.849 kb on - strand, within BWI76_RS05565 at 1165.880 kb on + strand, within BWI76_RS05565 at 1165.880 kb on + strand, within BWI76_RS05565 at 1165.880 kb on + strand, within BWI76_RS05565 at 1165.937 kb on + strand, within BWI76_RS05565 at 1166.292 kb on - strand, within BWI76_RS05565 at 1166.406 kb on - strand, within BWI76_RS05565 at 1166.526 kb on + strand at 1166.557 kb on - strand at 1166.782 kb on + strand, within BWI76_RS05570 at 1166.981 kb on + strand at 1167.207 kb on - strand, within BWI76_RS05575 at 1167.224 kb on + strand, within BWI76_RS05575 at 1167.225 kb on + strand, within BWI76_RS05575 at 1167.250 kb on - strand, within BWI76_RS05575 at 1167.316 kb on + strand, within BWI76_RS05575
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,164,198 - +0.3 1,164,207 + +0.3 1,164,207 + -0.3 1,164,215 - +0.1 1,164,296 + BWI76_RS05555 0.27 +0.6 1,164,396 - BWI76_RS05555 0.52 -1.9 1,165,323 - BWI76_RS05565 0.11 +1.3 1,165,330 + BWI76_RS05565 0.11 -0.2 1,165,348 - BWI76_RS05565 0.12 +0.5 1,165,348 - BWI76_RS05565 0.12 -0.1 1,165,383 + BWI76_RS05565 0.15 +0.5 1,165,614 + BWI76_RS05565 0.31 +1.0 1,165,616 + BWI76_RS05565 0.31 +0.7 1,165,616 + BWI76_RS05565 0.31 +2.4 1,165,652 + BWI76_RS05565 0.34 +0.6 1,165,659 - BWI76_RS05565 0.34 +0.3 1,165,670 + BWI76_RS05565 0.35 +1.2 1,165,678 - BWI76_RS05565 0.36 +0.5 1,165,849 - BWI76_RS05565 0.48 +4.8 1,165,880 + BWI76_RS05565 0.50 -0.9 1,165,880 + BWI76_RS05565 0.50 +0.1 1,165,880 + BWI76_RS05565 0.50 +0.3 1,165,937 + BWI76_RS05565 0.54 -1.2 1,166,292 - BWI76_RS05565 0.79 -0.0 1,166,406 - BWI76_RS05565 0.87 +0.2 1,166,526 + -0.7 1,166,557 - -0.0 1,166,782 + BWI76_RS05570 0.46 +1.3 1,166,981 + +0.4 1,167,207 - BWI76_RS05575 0.19 +2.4 1,167,224 + BWI76_RS05575 0.20 -0.3 1,167,225 + BWI76_RS05575 0.20 +1.8 1,167,250 - BWI76_RS05575 0.22 +0.4 1,167,316 + BWI76_RS05575 0.28 +1.2
Or see this region's nucleotide sequence