Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02635 and BWI76_RS02640 are separated by 9 nucleotides BWI76_RS02640 and BWI76_RS02645 overlap by 1 nucleotides BWI76_RS02645 and BWI76_RS02650 are separated by 61 nucleotides
BWI76_RS02635: BWI76_RS02635 - 3-keto-L-gulonate-6-phosphate decarboxylase, at 538,266 to 538,916
_RS02635
BWI76_RS02640: BWI76_RS02640 - xylulose 5-phosphate 3-epimerase, at 538,926 to 539,780
_RS02640
BWI76_RS02645: BWI76_RS02645 - L-ribulose-5-phosphate 4-epimerase, at 539,780 to 540,466
_RS02645
BWI76_RS02650: BWI76_RS02650 - MFS transporter, at 540,528 to 541,721
_RS02650
Position (kb) 539
540
541 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 538.810 kb on - strand, within BWI76_RS02635 at 538.883 kb on + strand at 538.891 kb on - strand at 538.904 kb on + strand at 538.980 kb on - strand at 539.103 kb on - strand, within BWI76_RS02640 at 539.103 kb on - strand, within BWI76_RS02640 at 539.103 kb on - strand, within BWI76_RS02640 at 539.113 kb on - strand, within BWI76_RS02640 at 539.187 kb on - strand, within BWI76_RS02640 at 539.335 kb on + strand, within BWI76_RS02640 at 539.335 kb on + strand, within BWI76_RS02640 at 539.427 kb on + strand, within BWI76_RS02640 at 539.435 kb on - strand, within BWI76_RS02640 at 539.514 kb on - strand, within BWI76_RS02640 at 539.546 kb on - strand, within BWI76_RS02640 at 539.566 kb on + strand, within BWI76_RS02640 at 539.629 kb on + strand, within BWI76_RS02640 at 539.755 kb on + strand at 539.765 kb on + strand at 539.773 kb on - strand at 539.778 kb on - strand at 539.860 kb on + strand, within BWI76_RS02645 at 539.967 kb on + strand, within BWI76_RS02645 at 540.053 kb on + strand, within BWI76_RS02645 at 540.053 kb on + strand, within BWI76_RS02645 at 540.061 kb on - strand, within BWI76_RS02645 at 540.080 kb on + strand, within BWI76_RS02645 at 540.087 kb on - strand, within BWI76_RS02645 at 540.184 kb on + strand, within BWI76_RS02645 at 540.245 kb on - strand, within BWI76_RS02645 at 540.372 kb on + strand, within BWI76_RS02645 at 540.385 kb on + strand, within BWI76_RS02645 at 540.516 kb on - strand at 540.585 kb on + strand at 540.644 kb on + strand at 540.652 kb on - strand, within BWI76_RS02650 at 540.652 kb on - strand, within BWI76_RS02650 at 540.673 kb on - strand, within BWI76_RS02650 at 540.673 kb on - strand, within BWI76_RS02650 at 540.805 kb on + strand, within BWI76_RS02650 at 540.852 kb on - strand at 540.892 kb on - strand, within BWI76_RS02650 at 540.892 kb on - strand, within BWI76_RS02650 at 541.050 kb on + strand, within BWI76_RS02650 at 541.079 kb on + strand, within BWI76_RS02650 at 541.106 kb on - strand, within BWI76_RS02650 at 541.113 kb on - strand, within BWI76_RS02650 at 541.115 kb on + strand, within BWI76_RS02650 at 541.178 kb on + strand, within BWI76_RS02650 at 541.351 kb on - strand, within BWI76_RS02650 at 541.457 kb on + strand, within BWI76_RS02650 at 541.457 kb on + strand, within BWI76_RS02650 at 541.457 kb on + strand at 541.465 kb on - strand, within BWI76_RS02650 at 541.465 kb on - strand, within BWI76_RS02650 at 541.465 kb on - strand, within BWI76_RS02650
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 538,810 - BWI76_RS02635 0.84 -2.9 538,883 + +0.7 538,891 - +1.8 538,904 + -0.2 538,980 - +0.5 539,103 - BWI76_RS02640 0.21 +0.0 539,103 - BWI76_RS02640 0.21 +0.2 539,103 - BWI76_RS02640 0.21 -0.4 539,113 - BWI76_RS02640 0.22 +0.1 539,187 - BWI76_RS02640 0.31 +0.1 539,335 + BWI76_RS02640 0.48 -0.0 539,335 + BWI76_RS02640 0.48 +1.1 539,427 + BWI76_RS02640 0.59 +1.7 539,435 - BWI76_RS02640 0.60 -1.2 539,514 - BWI76_RS02640 0.69 +1.1 539,546 - BWI76_RS02640 0.73 +0.1 539,566 + BWI76_RS02640 0.75 -0.1 539,629 + BWI76_RS02640 0.82 +1.1 539,755 + -0.2 539,765 + +0.6 539,773 - +0.6 539,778 - +0.3 539,860 + BWI76_RS02645 0.12 +0.0 539,967 + BWI76_RS02645 0.27 +0.3 540,053 + BWI76_RS02645 0.40 +2.4 540,053 + BWI76_RS02645 0.40 +0.6 540,061 - BWI76_RS02645 0.41 +0.5 540,080 + BWI76_RS02645 0.44 +0.0 540,087 - BWI76_RS02645 0.45 +1.6 540,184 + BWI76_RS02645 0.59 +0.4 540,245 - BWI76_RS02645 0.68 +2.1 540,372 + BWI76_RS02645 0.86 +0.2 540,385 + BWI76_RS02645 0.88 -0.4 540,516 - +0.4 540,585 + -0.4 540,644 + +0.3 540,652 - BWI76_RS02650 0.10 +0.5 540,652 - BWI76_RS02650 0.10 +1.0 540,673 - BWI76_RS02650 0.12 +1.7 540,673 - BWI76_RS02650 0.12 +0.4 540,805 + BWI76_RS02650 0.23 +0.6 540,852 - +0.1 540,892 - BWI76_RS02650 0.30 +1.1 540,892 - BWI76_RS02650 0.30 +1.7 541,050 + BWI76_RS02650 0.44 -0.2 541,079 + BWI76_RS02650 0.46 +0.2 541,106 - BWI76_RS02650 0.48 +0.2 541,113 - BWI76_RS02650 0.49 -0.1 541,115 + BWI76_RS02650 0.49 +0.0 541,178 + BWI76_RS02650 0.54 +0.1 541,351 - BWI76_RS02650 0.69 +0.4 541,457 + BWI76_RS02650 0.78 -2.9 541,457 + BWI76_RS02650 0.78 -0.3 541,457 + -0.9 541,465 - BWI76_RS02650 0.78 +0.5 541,465 - BWI76_RS02650 0.78 +0.9 541,465 - BWI76_RS02650 0.78 -0.2
Or see this region's nucleotide sequence