Experiment: RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01100 and BWI76_RS01105 are separated by 18 nucleotides BWI76_RS01105 and BWI76_RS01110 overlap by 23 nucleotides BWI76_RS01110 and BWI76_RS01115 are separated by 4 nucleotides
BWI76_RS01100: BWI76_RS01100 - dTDP-glucose 4,6-dehydratase, at 226,819 to 227,887
_RS01100
BWI76_RS01105: BWI76_RS01105 - glucose-1-phosphate thymidylyltransferase, at 227,906 to 228,787
_RS01105
BWI76_RS01110: BWI76_RS01110 - TDP-fucosamine acetyltransferase, at 228,765 to 229,439
_RS01110
BWI76_RS01115: BWI76_RS01115 - dTDP-4-amino-4,6-dideoxygalactose transaminase, at 229,444 to 230,574
_RS01115
Position (kb) 228
229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 227.921 kb on - strand at 228.196 kb on - strand, within BWI76_RS01105 at 228.249 kb on + strand, within BWI76_RS01105 at 228.263 kb on - strand, within BWI76_RS01105 at 228.273 kb on - strand, within BWI76_RS01105 at 228.384 kb on + strand, within BWI76_RS01105 at 228.654 kb on + strand, within BWI76_RS01105 at 228.789 kb on - strand at 228.839 kb on - strand, within BWI76_RS01110 at 228.927 kb on + strand, within BWI76_RS01110 at 229.276 kb on - strand, within BWI76_RS01110 at 229.279 kb on + strand, within BWI76_RS01110 at 229.315 kb on + strand, within BWI76_RS01110 at 229.536 kb on - strand at 229.602 kb on + strand, within BWI76_RS01115 at 229.711 kb on - strand, within BWI76_RS01115 at 229.716 kb on - strand, within BWI76_RS01115
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Dyella japonica UNC79MFTsu3.2 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 227,921 - +0.9 228,196 - BWI76_RS01105 0.33 -3.2 228,249 + BWI76_RS01105 0.39 +0.6 228,263 - BWI76_RS01105 0.40 -3.2 228,273 - BWI76_RS01105 0.42 -2.2 228,384 + BWI76_RS01105 0.54 -1.0 228,654 + BWI76_RS01105 0.85 -0.0 228,789 - -1.3 228,839 - BWI76_RS01110 0.11 -1.4 228,927 + BWI76_RS01110 0.24 +1.2 229,276 - BWI76_RS01110 0.76 -2.0 229,279 + BWI76_RS01110 0.76 -1.3 229,315 + BWI76_RS01110 0.81 -0.2 229,536 - -3.3 229,602 + BWI76_RS01115 0.14 -1.7 229,711 - BWI76_RS01115 0.24 -0.4 229,716 - BWI76_RS01115 0.24 -1.2
Or see this region's nucleotide sequence