Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27670 and BWI76_RS27675 overlap by 4 nucleotides BWI76_RS27675 and BWI76_RS27680 are separated by 80 nucleotides BWI76_RS27680 and BWI76_RS27685 are separated by 148 nucleotides
BWI76_RS27670: BWI76_RS27670 - LysR family transcriptional regulator, at 5,740,075 to 5,740,989
_RS27670
BWI76_RS27675: BWI76_RS27675 - EmrB/QacA family drug resistance transporter, at 5,740,986 to 5,742,482
_RS27675
BWI76_RS27680: BWI76_RS27680 - TetR family transcriptional regulator, at 5,742,563 to 5,743,210
_RS27680
BWI76_RS27685: BWI76_RS27685 - N-acetyltransferase, at 5,743,359 to 5,743,775
_RS27685
Position (kb) 5740
5741
5742
5743 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5740.167 kb on - strand, within BWI76_RS27670 at 5740.297 kb on + strand, within BWI76_RS27670 at 5740.356 kb on + strand, within BWI76_RS27670 at 5740.453 kb on - strand, within BWI76_RS27670 at 5740.884 kb on + strand, within BWI76_RS27670 at 5740.884 kb on + strand, within BWI76_RS27670 at 5740.910 kb on + strand at 5740.918 kb on - strand at 5740.968 kb on + strand at 5740.999 kb on + strand at 5741.031 kb on - strand at 5741.141 kb on - strand, within BWI76_RS27675 at 5741.217 kb on + strand, within BWI76_RS27675 at 5741.287 kb on - strand, within BWI76_RS27675 at 5741.296 kb on + strand, within BWI76_RS27675 at 5741.304 kb on - strand, within BWI76_RS27675 at 5741.417 kb on - strand, within BWI76_RS27675 at 5741.556 kb on + strand, within BWI76_RS27675 at 5741.561 kb on + strand, within BWI76_RS27675 at 5741.584 kb on + strand, within BWI76_RS27675 at 5741.594 kb on + strand, within BWI76_RS27675 at 5741.841 kb on + strand, within BWI76_RS27675 at 5742.099 kb on - strand, within BWI76_RS27675 at 5742.169 kb on - strand, within BWI76_RS27675 at 5742.284 kb on - strand, within BWI76_RS27675 at 5742.336 kb on - strand at 5742.477 kb on + strand at 5742.654 kb on + strand, within BWI76_RS27680 at 5742.668 kb on - strand, within BWI76_RS27680 at 5743.065 kb on + strand, within BWI76_RS27680 at 5743.073 kb on - strand, within BWI76_RS27680 at 5743.073 kb on - strand, within BWI76_RS27680 at 5743.073 kb on - strand, within BWI76_RS27680 at 5743.080 kb on + strand, within BWI76_RS27680 at 5743.170 kb on + strand at 5743.178 kb on - strand at 5743.229 kb on + strand at 5743.240 kb on + strand at 5743.247 kb on + strand at 5743.354 kb on - strand at 5743.354 kb on - strand at 5743.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 5,740,167 - BWI76_RS27670 0.10 +2.0 5,740,297 + BWI76_RS27670 0.24 +1.0 5,740,356 + BWI76_RS27670 0.31 +0.7 5,740,453 - BWI76_RS27670 0.41 +0.4 5,740,884 + BWI76_RS27670 0.88 -0.0 5,740,884 + BWI76_RS27670 0.88 +0.9 5,740,910 + +0.5 5,740,918 - -0.4 5,740,968 + -0.0 5,740,999 + -1.8 5,741,031 - +0.6 5,741,141 - BWI76_RS27675 0.10 +1.8 5,741,217 + BWI76_RS27675 0.15 +1.8 5,741,287 - BWI76_RS27675 0.20 +0.5 5,741,296 + BWI76_RS27675 0.21 +0.5 5,741,304 - BWI76_RS27675 0.21 -1.0 5,741,417 - BWI76_RS27675 0.29 +1.9 5,741,556 + BWI76_RS27675 0.38 +1.0 5,741,561 + BWI76_RS27675 0.38 +0.2 5,741,584 + BWI76_RS27675 0.40 +0.4 5,741,594 + BWI76_RS27675 0.41 +0.0 5,741,841 + BWI76_RS27675 0.57 +2.0 5,742,099 - BWI76_RS27675 0.74 +1.0 5,742,169 - BWI76_RS27675 0.79 +1.3 5,742,284 - BWI76_RS27675 0.87 +0.2 5,742,336 - +1.0 5,742,477 + +1.0 5,742,654 + BWI76_RS27680 0.14 +0.4 5,742,668 - BWI76_RS27680 0.16 -0.2 5,743,065 + BWI76_RS27680 0.77 -0.5 5,743,073 - BWI76_RS27680 0.79 -2.5 5,743,073 - BWI76_RS27680 0.79 -3.5 5,743,073 - BWI76_RS27680 0.79 -0.7 5,743,080 + BWI76_RS27680 0.80 -1.4 5,743,170 + -0.3 5,743,178 - -0.0 5,743,229 + +1.0 5,743,240 + +0.4 5,743,247 + +1.2 5,743,354 - +0.6 5,743,354 - +0.8 5,743,396 - -0.6
Or see this region's nucleotide sequence