Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24730

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBWI76_RS24725 and BWI76_RS24730 are separated by 18 nucleotidesBWI76_RS24730 and BWI76_RS24735 are separated by 482 nucleotides BWI76_RS24725: BWI76_RS24725 - altronate hydrolase, at 5,155,999 to 5,157,486 _RS24725 BWI76_RS24730: BWI76_RS24730 - uronate isomerase, at 5,157,505 to 5,158,917 _RS24730 BWI76_RS24735: BWI76_RS24735 - MFS transporter, at 5,159,400 to 5,160,701 _RS24735 Position (kb) 5157 5158 5159Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5156.827 kb on - strand, within BWI76_RS24725at 5156.901 kb on - strand, within BWI76_RS24725at 5157.201 kb on + strand, within BWI76_RS24725at 5157.351 kb on + strandat 5157.359 kb on - strandat 5157.359 kb on - strandat 5157.359 kb on - strandat 5157.359 kb on - strandat 5157.417 kb on - strandat 5157.483 kb on + strandat 5157.659 kb on + strand, within BWI76_RS24730at 5157.713 kb on + strand, within BWI76_RS24730at 5157.903 kb on - strand, within BWI76_RS24730at 5158.062 kb on - strand, within BWI76_RS24730at 5158.088 kb on + strand, within BWI76_RS24730at 5158.259 kb on + strand, within BWI76_RS24730at 5158.353 kb on - strand, within BWI76_RS24730at 5158.939 kb on + strandat 5159.208 kb on + strandat 5159.314 kb on - strandat 5159.474 kb on + strandat 5159.482 kb on - strandat 5159.566 kb on - strand, within BWI76_RS24735at 5159.575 kb on + strand, within BWI76_RS24735at 5159.585 kb on + strand, within BWI76_RS24735at 5159.663 kb on - strand, within BWI76_RS24735

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
remove
5,156,827 - BWI76_RS24725 0.56 -1.7
5,156,901 - BWI76_RS24725 0.61 +0.2
5,157,201 + BWI76_RS24725 0.81 -0.3
5,157,351 + +0.9
5,157,359 - +2.6
5,157,359 - -1.0
5,157,359 - +0.6
5,157,359 - +0.2
5,157,417 - +0.2
5,157,483 + +0.4
5,157,659 + BWI76_RS24730 0.11 +0.7
5,157,713 + BWI76_RS24730 0.15 +0.4
5,157,903 - BWI76_RS24730 0.28 -0.0
5,158,062 - BWI76_RS24730 0.39 +1.8
5,158,088 + BWI76_RS24730 0.41 +0.4
5,158,259 + BWI76_RS24730 0.53 +0.7
5,158,353 - BWI76_RS24730 0.60 +2.0
5,158,939 + +0.3
5,159,208 + -3.1
5,159,314 - +0.6
5,159,474 + -3.4
5,159,482 - +0.7
5,159,566 - BWI76_RS24735 0.13 +1.4
5,159,575 + BWI76_RS24735 0.13 +0.5
5,159,585 + BWI76_RS24735 0.14 -0.6
5,159,663 - BWI76_RS24735 0.20 -0.9

Or see this region's nucleotide sequence