Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22845 and BWI76_RS22850 are separated by 2 nucleotides BWI76_RS22850 and BWI76_RS22855 overlap by 1 nucleotides BWI76_RS22855 and BWI76_RS22860 are separated by 633 nucleotides
BWI76_RS22845: BWI76_RS22845 - flavodoxin, at 4,767,099 to 4,767,542
_RS22845
BWI76_RS22850: BWI76_RS22850 - tRNA pseudouridine synthase TruC, at 4,767,545 to 4,768,339
_RS22850
BWI76_RS22855: BWI76_RS22855 - hypothetical protein, at 4,768,339 to 4,768,668
_RS22855
BWI76_RS22860: BWI76_RS22860 - SecY-interacting protein, at 4,769,302 to 4,769,847
_RS22860
Position (kb) 4768
4769 Strain fitness (log2 ratio) -1
0
1
2 at 4767.408 kb on + strand, within BWI76_RS22845 at 4767.889 kb on + strand, within BWI76_RS22850 at 4768.137 kb on + strand, within BWI76_RS22850 at 4768.291 kb on - strand at 4768.370 kb on + strand at 4768.428 kb on + strand, within BWI76_RS22855 at 4768.530 kb on + strand, within BWI76_RS22855 at 4768.538 kb on - strand, within BWI76_RS22855 at 4768.610 kb on - strand, within BWI76_RS22855 at 4768.748 kb on - strand at 4768.748 kb on - strand at 4768.803 kb on - strand at 4768.859 kb on - strand at 4768.902 kb on - strand at 4768.904 kb on + strand at 4768.985 kb on - strand at 4769.116 kb on + strand at 4769.378 kb on - strand, within BWI76_RS22860
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,767,408 + BWI76_RS22845 0.70 +0.3 4,767,889 + BWI76_RS22850 0.43 +0.4 4,768,137 + BWI76_RS22850 0.74 +0.1 4,768,291 - +0.3 4,768,370 + -1.1 4,768,428 + BWI76_RS22855 0.27 -0.8 4,768,530 + BWI76_RS22855 0.58 +2.0 4,768,538 - BWI76_RS22855 0.60 +0.0 4,768,610 - BWI76_RS22855 0.82 -0.2 4,768,748 - +1.2 4,768,748 - -0.7 4,768,803 - +1.1 4,768,859 - +1.9 4,768,902 - +0.8 4,768,904 + +0.2 4,768,985 - +1.1 4,769,116 + +1.1 4,769,378 - BWI76_RS22860 0.14 +0.0
Or see this region's nucleotide sequence