Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21910 and BWI76_RS21915 are separated by 74 nucleotides BWI76_RS21915 and BWI76_RS21920 are separated by 6 nucleotides BWI76_RS21920 and BWI76_RS21925 are separated by 19 nucleotides
BWI76_RS21910: BWI76_RS21910 - glyoxylate/hydroxypyruvate reductase A, at 4,578,260 to 4,579,192
_RS21910
BWI76_RS21915: BWI76_RS21915 - short-chain dehydrogenase/reductase SDR, at 4,579,267 to 4,580,061
_RS21915
BWI76_RS21920: BWI76_RS21920 - amino acid ABC transporter substrate-binding protein, at 4,580,068 to 4,580,284
_RS21920
BWI76_RS21925: BWI76_RS21925 - agmatinase, at 4,580,304 to 4,581,275
_RS21925
Position (kb)
4579
4580
4581 Strain fitness (log2 ratio)
-1
0
1
2 at 4578.296 kb on + strand at 4578.304 kb on - strand at 4578.342 kb on - strand at 4578.700 kb on + strand, within BWI76_RS21910 at 4578.843 kb on - strand, within BWI76_RS21910 at 4578.855 kb on - strand, within BWI76_RS21910 at 4579.229 kb on + strand at 4579.405 kb on + strand, within BWI76_RS21915 at 4579.405 kb on + strand, within BWI76_RS21915 at 4579.660 kb on + strand, within BWI76_RS21915 at 4579.683 kb on - strand, within BWI76_RS21915 at 4579.991 kb on + strand at 4580.000 kb on + strand at 4580.073 kb on + strand at 4580.143 kb on - strand, within BWI76_RS21920 at 4580.145 kb on - strand, within BWI76_RS21920 at 4580.152 kb on + strand, within BWI76_RS21920 at 4580.152 kb on + strand, within BWI76_RS21920 at 4580.595 kb on + strand, within BWI76_RS21925 at 4580.630 kb on + strand, within BWI76_RS21925 at 4580.673 kb on + strand, within BWI76_RS21925 at 4580.673 kb on - strand, within BWI76_RS21925 at 4580.998 kb on + strand, within BWI76_RS21925
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,578,296 + -1.2 4,578,304 - +0.7 4,578,342 - +0.8 4,578,700 + BWI76_RS21910 0.47 -0.2 4,578,843 - BWI76_RS21910 0.62 +0.4 4,578,855 - BWI76_RS21910 0.64 +0.4 4,579,229 + +0.1 4,579,405 + BWI76_RS21915 0.17 +1.1 4,579,405 + BWI76_RS21915 0.17 +0.5 4,579,660 + BWI76_RS21915 0.49 +1.8 4,579,683 - BWI76_RS21915 0.52 +0.5 4,579,991 + -0.2 4,580,000 + +0.8 4,580,073 + +1.2 4,580,143 - BWI76_RS21920 0.35 +0.7 4,580,145 - BWI76_RS21920 0.35 -0.7 4,580,152 + BWI76_RS21920 0.39 +0.8 4,580,152 + BWI76_RS21920 0.39 +0.1 4,580,595 + BWI76_RS21925 0.30 -1.6 4,580,630 + BWI76_RS21925 0.34 +0.4 4,580,673 + BWI76_RS21925 0.38 +1.0 4,580,673 - BWI76_RS21925 0.38 +1.0 4,580,998 + BWI76_RS21925 0.71 +0.0
Or see this region's nucleotide sequence