Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20750 and BWI76_RS20755 are separated by 10 nucleotides BWI76_RS20755 and BWI76_RS20760 are separated by 9 nucleotides BWI76_RS20760 and BWI76_RS20765 are separated by 20 nucleotides
BWI76_RS20750: BWI76_RS20750 - carboxysome structural protein EutK, at 4,337,612 to 4,338,103
_RS20750
BWI76_RS20755: BWI76_RS20755 - microcompartment protein EutL, at 4,338,114 to 4,338,773
_RS20755
BWI76_RS20760: BWI76_RS20760 - ethanolamine ammonia-lyase small subunit, at 4,338,783 to 4,339,676
_RS20760
BWI76_RS20765: BWI76_RS20765 - ethanolamine ammonia lyase large subunit, at 4,339,697 to 4,341,058
_RS20765
Position (kb) 4338
4339
4340 Strain fitness (log2 ratio) -1
0
1
2 at 4337.969 kb on - strand, within BWI76_RS20750 at 4338.257 kb on - strand, within BWI76_RS20755 at 4338.576 kb on + strand, within BWI76_RS20755 at 4338.587 kb on - strand, within BWI76_RS20755 at 4338.667 kb on - strand, within BWI76_RS20755 at 4338.714 kb on + strand at 4339.259 kb on - strand, within BWI76_RS20760 at 4339.278 kb on - strand, within BWI76_RS20760 at 4339.440 kb on - strand, within BWI76_RS20760 at 4339.558 kb on + strand, within BWI76_RS20760 at 4339.843 kb on + strand, within BWI76_RS20765 at 4339.957 kb on + strand, within BWI76_RS20765 at 4340.401 kb on - strand, within BWI76_RS20765 at 4340.450 kb on + strand, within BWI76_RS20765 at 4340.585 kb on + strand, within BWI76_RS20765 at 4340.649 kb on + strand, within BWI76_RS20765
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,337,969 - BWI76_RS20750 0.73 +0.8 4,338,257 - BWI76_RS20755 0.22 -0.1 4,338,576 + BWI76_RS20755 0.70 +0.3 4,338,587 - BWI76_RS20755 0.72 -0.5 4,338,667 - BWI76_RS20755 0.84 -1.5 4,338,714 + +1.9 4,339,259 - BWI76_RS20760 0.53 +2.6 4,339,278 - BWI76_RS20760 0.55 +1.4 4,339,440 - BWI76_RS20760 0.73 +1.1 4,339,558 + BWI76_RS20760 0.87 -0.0 4,339,843 + BWI76_RS20765 0.11 +0.5 4,339,957 + BWI76_RS20765 0.19 +0.9 4,340,401 - BWI76_RS20765 0.52 +1.8 4,340,450 + BWI76_RS20765 0.55 -0.2 4,340,585 + BWI76_RS20765 0.65 +0.1 4,340,649 + BWI76_RS20765 0.70 +0.7
Or see this region's nucleotide sequence