Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19455 and BWI76_RS19460 are separated by 12 nucleotides BWI76_RS19460 and BWI76_RS19465 are separated by 110 nucleotides BWI76_RS19465 and BWI76_RS19470 are separated by 53 nucleotides
BWI76_RS19455: BWI76_RS19455 - hypothetical protein, at 4,065,632 to 4,067,638
_RS19455
BWI76_RS19460: BWI76_RS19460 - lipoprotein, at 4,067,651 to 4,068,112
_RS19460
BWI76_RS19465: BWI76_RS19465 - hypothetical protein, at 4,068,223 to 4,068,690
_RS19465
BWI76_RS19470: BWI76_RS19470 - DNA-binding response regulator, at 4,068,744 to 4,069,463
_RS19470
Position (kb) 4067
4068
4069 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4066.931 kb on - strand, within BWI76_RS19455 at 4067.141 kb on + strand, within BWI76_RS19455 at 4067.168 kb on + strand, within BWI76_RS19455 at 4067.226 kb on - strand, within BWI76_RS19455 at 4067.320 kb on + strand, within BWI76_RS19455 at 4067.431 kb on + strand, within BWI76_RS19455 at 4067.613 kb on - strand at 4067.766 kb on - strand, within BWI76_RS19460 at 4067.785 kb on - strand, within BWI76_RS19460 at 4067.824 kb on + strand, within BWI76_RS19460 at 4067.911 kb on - strand, within BWI76_RS19460 at 4068.120 kb on - strand at 4068.441 kb on - strand, within BWI76_RS19465 at 4068.528 kb on + strand, within BWI76_RS19465 at 4068.530 kb on - strand, within BWI76_RS19465 at 4068.653 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 4,066,931 - BWI76_RS19455 0.65 +1.6 4,067,141 + BWI76_RS19455 0.75 -0.0 4,067,168 + BWI76_RS19455 0.77 +0.8 4,067,226 - BWI76_RS19455 0.79 -0.0 4,067,320 + BWI76_RS19455 0.84 +3.2 4,067,431 + BWI76_RS19455 0.90 -0.8 4,067,613 - -0.3 4,067,766 - BWI76_RS19460 0.25 -2.9 4,067,785 - BWI76_RS19460 0.29 -1.6 4,067,824 + BWI76_RS19460 0.37 -0.3 4,067,911 - BWI76_RS19460 0.56 -0.6 4,068,120 - +1.9 4,068,441 - BWI76_RS19465 0.47 -2.9 4,068,528 + BWI76_RS19465 0.65 -2.3 4,068,530 - BWI76_RS19465 0.66 +1.1 4,068,653 + -2.6
Or see this region's nucleotide sequence