Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18840 and BWI76_RS18845 overlap by 14 nucleotides BWI76_RS18845 and BWI76_RS18850 are separated by 195 nucleotides BWI76_RS18850 and BWI76_RS18855 are separated by 30 nucleotides
BWI76_RS18840: BWI76_RS18840 - nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, at 3,907,993 to 3,909,378
_RS18840
BWI76_RS18845: BWI76_RS18845 - nitrogen fixation protein NifX, at 3,909,365 to 3,909,835
_RS18845
BWI76_RS18850: BWI76_RS18850 - iron-sulfur cluster assembly scaffold protein NifU, at 3,910,031 to 3,910,855
_RS18850
BWI76_RS18855: BWI76_RS18855 - cysteine desulfurase NifS, at 3,910,886 to 3,912,088
_RS18855
Position (kb)
3910
3911 Strain fitness (log2 ratio)
-1
0
1
2 at 3909.394 kb on + strand at 3909.471 kb on - strand at 3909.538 kb on - strand, within BWI76_RS18845 at 3909.610 kb on + strand, within BWI76_RS18845 at 3909.735 kb on + strand, within BWI76_RS18845 at 3910.023 kb on - strand at 3910.273 kb on + strand, within BWI76_RS18850 at 3910.355 kb on - strand, within BWI76_RS18850 at 3910.481 kb on + strand, within BWI76_RS18850 at 3910.481 kb on + strand, within BWI76_RS18850 at 3910.481 kb on + strand, within BWI76_RS18850 at 3910.489 kb on - strand, within BWI76_RS18850 at 3910.709 kb on + strand, within BWI76_RS18850 at 3910.712 kb on - strand, within BWI76_RS18850 at 3910.782 kb on + strand at 3911.309 kb on + strand, within BWI76_RS18855 at 3911.517 kb on + strand, within BWI76_RS18855 at 3911.567 kb on + strand, within BWI76_RS18855 at 3911.838 kb on + strand, within BWI76_RS18855
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,909,394 + -1.4 3,909,471 - -0.2 3,909,538 - BWI76_RS18845 0.37 +1.3 3,909,610 + BWI76_RS18845 0.52 +0.2 3,909,735 + BWI76_RS18845 0.79 +0.0 3,910,023 - +0.0 3,910,273 + BWI76_RS18850 0.29 +0.4 3,910,355 - BWI76_RS18850 0.39 +0.8 3,910,481 + BWI76_RS18850 0.55 +1.4 3,910,481 + BWI76_RS18850 0.55 +0.4 3,910,481 + BWI76_RS18850 0.55 +1.3 3,910,489 - BWI76_RS18850 0.56 -0.1 3,910,709 + BWI76_RS18850 0.82 +1.0 3,910,712 - BWI76_RS18850 0.83 +0.0 3,910,782 + +1.0 3,911,309 + BWI76_RS18855 0.35 -0.2 3,911,517 + BWI76_RS18855 0.52 +0.4 3,911,567 + BWI76_RS18855 0.57 +0.4 3,911,838 + BWI76_RS18855 0.79 +2.3
Or see this region's nucleotide sequence