Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18125 and BWI76_RS18130 are separated by 15 nucleotides BWI76_RS18130 and BWI76_RS18135 are separated by 78 nucleotides BWI76_RS18135 and BWI76_RS18140 overlap by 1 nucleotides
BWI76_RS18125: BWI76_RS18125 - murein DD-endopeptidase MepM, at 3,767,313 to 3,768,635
_RS18125
BWI76_RS18130: BWI76_RS18130 - zinc ABC transporter substrate-binding protein, at 3,768,651 to 3,769,595
_RS18130
BWI76_RS18135: BWI76_RS18135 - zinc ABC transporter ATP-binding protein ZnuC, at 3,769,674 to 3,770,426
_RS18135
BWI76_RS18140: BWI76_RS18140 - zinc ABC transporter permease, at 3,770,426 to 3,771,211
_RS18140
Position (kb)
3768
3769
3770 Strain fitness (log2 ratio)
-2
-1
0
1 at 3767.853 kb on - strand, within BWI76_RS18125 at 3768.428 kb on + strand, within BWI76_RS18125 at 3768.489 kb on - strand, within BWI76_RS18125 at 3769.014 kb on - strand, within BWI76_RS18130 at 3769.014 kb on - strand, within BWI76_RS18130 at 3769.370 kb on - strand, within BWI76_RS18130 at 3769.758 kb on - strand, within BWI76_RS18135 at 3769.758 kb on - strand, within BWI76_RS18135 at 3769.778 kb on + strand, within BWI76_RS18135 at 3769.890 kb on - strand, within BWI76_RS18135 at 3769.991 kb on - strand, within BWI76_RS18135 at 3770.110 kb on + strand, within BWI76_RS18135 at 3770.122 kb on + strand, within BWI76_RS18135 at 3770.542 kb on - strand, within BWI76_RS18140 at 3770.578 kb on + strand, within BWI76_RS18140
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,767,853 - BWI76_RS18125 0.41 +0.0 3,768,428 + BWI76_RS18125 0.84 -0.9 3,768,489 - BWI76_RS18125 0.89 -1.9 3,769,014 - BWI76_RS18130 0.38 +1.1 3,769,014 - BWI76_RS18130 0.38 +0.6 3,769,370 - BWI76_RS18130 0.76 +0.8 3,769,758 - BWI76_RS18135 0.11 +1.3 3,769,758 - BWI76_RS18135 0.11 +0.3 3,769,778 + BWI76_RS18135 0.14 +1.3 3,769,890 - BWI76_RS18135 0.29 +0.8 3,769,991 - BWI76_RS18135 0.42 +0.3 3,770,110 + BWI76_RS18135 0.58 +0.6 3,770,122 + BWI76_RS18135 0.59 -2.4 3,770,542 - BWI76_RS18140 0.15 -1.4 3,770,578 + BWI76_RS18140 0.19 +0.4
Or see this region's nucleotide sequence