Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18130

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18125 and BWI76_RS18130 are separated by 15 nucleotidesBWI76_RS18130 and BWI76_RS18135 are separated by 78 nucleotidesBWI76_RS18135 and BWI76_RS18140 overlap by 1 nucleotides BWI76_RS18125: BWI76_RS18125 - murein DD-endopeptidase MepM, at 3,767,313 to 3,768,635 _RS18125 BWI76_RS18130: BWI76_RS18130 - zinc ABC transporter substrate-binding protein, at 3,768,651 to 3,769,595 _RS18130 BWI76_RS18135: BWI76_RS18135 - zinc ABC transporter ATP-binding protein ZnuC, at 3,769,674 to 3,770,426 _RS18135 BWI76_RS18140: BWI76_RS18140 - zinc ABC transporter permease, at 3,770,426 to 3,771,211 _RS18140 Position (kb) 3768 3769 3770Strain fitness (log2 ratio) -2 -1 0 1at 3767.853 kb on - strand, within BWI76_RS18125at 3768.428 kb on + strand, within BWI76_RS18125at 3768.489 kb on - strand, within BWI76_RS18125at 3769.014 kb on - strand, within BWI76_RS18130at 3769.014 kb on - strand, within BWI76_RS18130at 3769.370 kb on - strand, within BWI76_RS18130at 3769.758 kb on - strand, within BWI76_RS18135at 3769.758 kb on - strand, within BWI76_RS18135at 3769.778 kb on + strand, within BWI76_RS18135at 3769.890 kb on - strand, within BWI76_RS18135at 3769.991 kb on - strand, within BWI76_RS18135at 3770.110 kb on + strand, within BWI76_RS18135at 3770.122 kb on + strand, within BWI76_RS18135at 3770.542 kb on - strand, within BWI76_RS18140at 3770.578 kb on + strand, within BWI76_RS18140

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,767,853 - BWI76_RS18125 0.41 +0.0
3,768,428 + BWI76_RS18125 0.84 -0.9
3,768,489 - BWI76_RS18125 0.89 -1.9
3,769,014 - BWI76_RS18130 0.38 +1.1
3,769,014 - BWI76_RS18130 0.38 +0.6
3,769,370 - BWI76_RS18130 0.76 +0.8
3,769,758 - BWI76_RS18135 0.11 +1.3
3,769,758 - BWI76_RS18135 0.11 +0.3
3,769,778 + BWI76_RS18135 0.14 +1.3
3,769,890 - BWI76_RS18135 0.29 +0.8
3,769,991 - BWI76_RS18135 0.42 +0.3
3,770,110 + BWI76_RS18135 0.58 +0.6
3,770,122 + BWI76_RS18135 0.59 -2.4
3,770,542 - BWI76_RS18140 0.15 -1.4
3,770,578 + BWI76_RS18140 0.19 +0.4

Or see this region's nucleotide sequence