Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16680 and BWI76_RS16685 are separated by 42 nucleotides BWI76_RS16685 and BWI76_RS16690 overlap by 1 nucleotides BWI76_RS16690 and BWI76_RS16695 overlap by 4 nucleotides
BWI76_RS16680: BWI76_RS16680 - ABC transporter substrate-binding protein, at 3,451,896 to 3,453,599
_RS16680
BWI76_RS16685: BWI76_RS16685 - peptide ABC transporter permease, at 3,453,642 to 3,454,601
_RS16685
BWI76_RS16690: BWI76_RS16690 - peptide ABC transporter permease, at 3,454,601 to 3,455,548
_RS16690
BWI76_RS16695: BWI76_RS16695 - dipeptide/oligopeptide/nickel ABC transporter ATP-binding protein, at 3,455,545 to 3,456,525
_RS16695
Position (kb) 3453
3454
3455 Strain fitness (log2 ratio) -1
0
1
2 at 3452.687 kb on + strand, within BWI76_RS16680 at 3452.963 kb on + strand, within BWI76_RS16680 at 3453.141 kb on - strand, within BWI76_RS16680 at 3453.176 kb on + strand, within BWI76_RS16680 at 3453.767 kb on - strand, within BWI76_RS16685 at 3454.365 kb on - strand, within BWI76_RS16685 at 3454.377 kb on - strand at 3454.628 kb on - strand at 3454.952 kb on - strand, within BWI76_RS16690 at 3455.202 kb on + strand, within BWI76_RS16690 at 3455.311 kb on + strand, within BWI76_RS16690
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,452,687 + BWI76_RS16680 0.46 +0.2 3,452,963 + BWI76_RS16680 0.63 +0.0 3,453,141 - BWI76_RS16680 0.73 -0.8 3,453,176 + BWI76_RS16680 0.75 +0.0 3,453,767 - BWI76_RS16685 0.13 +2.4 3,454,365 - BWI76_RS16685 0.75 +0.6 3,454,377 - -0.3 3,454,628 - +0.5 3,454,952 - BWI76_RS16690 0.37 -0.2 3,455,202 + BWI76_RS16690 0.63 +0.1 3,455,311 + BWI76_RS16690 0.75 +0.3
Or see this region's nucleotide sequence