Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16290 and BWI76_RS16295 are separated by 174 nucleotides BWI76_RS16295 and BWI76_RS16300 are separated by 48 nucleotides
BWI76_RS16290: BWI76_RS16290 - Mal regulon transcriptional regulator MalI, at 3,381,335 to 3,382,363
_RS16290
BWI76_RS16295: BWI76_RS16295 - bifunctional PTS system maltose and glucose-specific transporter subunits IICB, at 3,382,538 to 3,384,130
_RS16295
BWI76_RS16300: BWI76_RS16300 - bifunctional beta-cystathionase/maltose regulon regulatory protein, at 3,384,179 to 3,385,357
_RS16300
Position (kb)
3382
3383
3384
3385 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3381.580 kb on + strand, within BWI76_RS16290 at 3382.293 kb on - strand at 3382.358 kb on + strand at 3382.599 kb on + strand at 3382.606 kb on - strand at 3382.624 kb on - strand at 3382.666 kb on - strand at 3382.721 kb on + strand, within BWI76_RS16295 at 3382.932 kb on - strand, within BWI76_RS16295 at 3383.087 kb on - strand, within BWI76_RS16295 at 3383.188 kb on - strand, within BWI76_RS16295 at 3383.362 kb on - strand, within BWI76_RS16295 at 3383.627 kb on - strand, within BWI76_RS16295 at 3383.693 kb on - strand, within BWI76_RS16295 at 3383.890 kb on - strand, within BWI76_RS16295 at 3383.966 kb on - strand, within BWI76_RS16295 at 3384.044 kb on - strand at 3384.074 kb on + strand at 3384.219 kb on + strand at 3384.219 kb on + strand at 3384.710 kb on + strand, within BWI76_RS16300 at 3384.859 kb on + strand, within BWI76_RS16300 at 3384.859 kb on + strand, within BWI76_RS16300
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,381,580 + BWI76_RS16290 0.24 +2.2 3,382,293 - +0.7 3,382,358 + -0.3 3,382,599 + -1.0 3,382,606 - -0.1 3,382,624 - -1.8 3,382,666 - -4.3 3,382,721 + BWI76_RS16295 0.11 -0.1 3,382,932 - BWI76_RS16295 0.25 -0.3 3,383,087 - BWI76_RS16295 0.34 -0.8 3,383,188 - BWI76_RS16295 0.41 +1.3 3,383,362 - BWI76_RS16295 0.52 +1.0 3,383,627 - BWI76_RS16295 0.68 +0.1 3,383,693 - BWI76_RS16295 0.73 +0.3 3,383,890 - BWI76_RS16295 0.85 +0.9 3,383,966 - BWI76_RS16295 0.90 -0.3 3,384,044 - -2.3 3,384,074 + +0.4 3,384,219 + -0.1 3,384,219 + +0.7 3,384,710 + BWI76_RS16300 0.45 -0.1 3,384,859 + BWI76_RS16300 0.58 +0.9 3,384,859 + BWI76_RS16300 0.58 +1.9
Or see this region's nucleotide sequence