Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16295

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16290 and BWI76_RS16295 are separated by 174 nucleotidesBWI76_RS16295 and BWI76_RS16300 are separated by 48 nucleotides BWI76_RS16290: BWI76_RS16290 - Mal regulon transcriptional regulator MalI, at 3,381,335 to 3,382,363 _RS16290 BWI76_RS16295: BWI76_RS16295 - bifunctional PTS system maltose and glucose-specific transporter subunits IICB, at 3,382,538 to 3,384,130 _RS16295 BWI76_RS16300: BWI76_RS16300 - bifunctional beta-cystathionase/maltose regulon regulatory protein, at 3,384,179 to 3,385,357 _RS16300 Position (kb) 3382 3383 3384 3385Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3381.580 kb on + strand, within BWI76_RS16290at 3382.293 kb on - strandat 3382.358 kb on + strandat 3382.599 kb on + strandat 3382.606 kb on - strandat 3382.624 kb on - strandat 3382.666 kb on - strandat 3382.721 kb on + strand, within BWI76_RS16295at 3382.932 kb on - strand, within BWI76_RS16295at 3383.087 kb on - strand, within BWI76_RS16295at 3383.188 kb on - strand, within BWI76_RS16295at 3383.362 kb on - strand, within BWI76_RS16295at 3383.627 kb on - strand, within BWI76_RS16295at 3383.693 kb on - strand, within BWI76_RS16295at 3383.890 kb on - strand, within BWI76_RS16295at 3383.966 kb on - strand, within BWI76_RS16295at 3384.044 kb on - strandat 3384.074 kb on + strandat 3384.219 kb on + strandat 3384.219 kb on + strandat 3384.710 kb on + strand, within BWI76_RS16300at 3384.859 kb on + strand, within BWI76_RS16300at 3384.859 kb on + strand, within BWI76_RS16300

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,381,580 + BWI76_RS16290 0.24 +2.2
3,382,293 - +0.7
3,382,358 + -0.3
3,382,599 + -1.0
3,382,606 - -0.1
3,382,624 - -1.8
3,382,666 - -4.3
3,382,721 + BWI76_RS16295 0.11 -0.1
3,382,932 - BWI76_RS16295 0.25 -0.3
3,383,087 - BWI76_RS16295 0.34 -0.8
3,383,188 - BWI76_RS16295 0.41 +1.3
3,383,362 - BWI76_RS16295 0.52 +1.0
3,383,627 - BWI76_RS16295 0.68 +0.1
3,383,693 - BWI76_RS16295 0.73 +0.3
3,383,890 - BWI76_RS16295 0.85 +0.9
3,383,966 - BWI76_RS16295 0.90 -0.3
3,384,044 - -2.3
3,384,074 + +0.4
3,384,219 + -0.1
3,384,219 + +0.7
3,384,710 + BWI76_RS16300 0.45 -0.1
3,384,859 + BWI76_RS16300 0.58 +0.9
3,384,859 + BWI76_RS16300 0.58 +1.9

Or see this region's nucleotide sequence