Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16095

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16085 and BWI76_RS16090 overlap by 4 nucleotidesBWI76_RS16090 and BWI76_RS16095 overlap by 20 nucleotidesBWI76_RS16095 and BWI76_RS16100 are separated by 67 nucleotides BWI76_RS16085: BWI76_RS16085 - two-component system sensor histidine kinase DcuS, at 3,335,222 to 3,336,841 _RS16085 BWI76_RS16090: BWI76_RS16090 - two-component system response regulator DcuR, at 3,336,838 to 3,337,557 _RS16090 BWI76_RS16095: BWI76_RS16095 - thiamine biosynthesis protein ApbE, at 3,337,538 to 3,338,488 _RS16095 BWI76_RS16100: BWI76_RS16100 - flavocytochrome c, at 3,338,556 to 3,341,333 _RS16100 Position (kb) 3337 3338 3339Strain fitness (log2 ratio) -2 -1 0 1at 3336.568 kb on + strand, within BWI76_RS16085at 3336.650 kb on + strand, within BWI76_RS16085at 3336.822 kb on + strandat 3337.028 kb on - strand, within BWI76_RS16090at 3337.119 kb on - strand, within BWI76_RS16090at 3337.143 kb on - strand, within BWI76_RS16090at 3337.825 kb on + strand, within BWI76_RS16095at 3338.170 kb on + strand, within BWI76_RS16095at 3338.331 kb on + strand, within BWI76_RS16095at 3338.426 kb on - strandat 3338.468 kb on - strandat 3338.505 kb on - strandat 3338.606 kb on - strandat 3339.005 kb on + strand, within BWI76_RS16100at 3339.415 kb on - strand, within BWI76_RS16100at 3339.439 kb on + strand, within BWI76_RS16100at 3339.465 kb on - strand, within BWI76_RS16100

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,336,568 + BWI76_RS16085 0.83 +0.9
3,336,650 + BWI76_RS16085 0.88 -2.3
3,336,822 + +0.1
3,337,028 - BWI76_RS16090 0.26 -1.5
3,337,119 - BWI76_RS16090 0.39 -1.5
3,337,143 - BWI76_RS16090 0.42 -0.1
3,337,825 + BWI76_RS16095 0.30 +0.8
3,338,170 + BWI76_RS16095 0.66 +1.2
3,338,331 + BWI76_RS16095 0.83 +0.8
3,338,426 - +1.1
3,338,468 - -0.2
3,338,505 - +0.8
3,338,606 - +0.3
3,339,005 + BWI76_RS16100 0.16 +1.1
3,339,415 - BWI76_RS16100 0.31 +0.1
3,339,439 + BWI76_RS16100 0.32 +1.6
3,339,465 - BWI76_RS16100 0.33 +0.2

Or see this region's nucleotide sequence