Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15485

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15475 and BWI76_RS15480 are separated by 90 nucleotidesBWI76_RS15480 and BWI76_RS15485 are separated by 51 nucleotidesBWI76_RS15485 and BWI76_RS15490 are separated by 278 nucleotides BWI76_RS15475: BWI76_RS15475 - NAD(P)-dependent oxidoreductase, at 3,242,553 to 3,243,302 _RS15475 BWI76_RS15480: BWI76_RS15480 - GntR family transcriptional regulator, at 3,243,393 to 3,244,079 _RS15480 BWI76_RS15485: BWI76_RS15485 - putative universal stress protein G, at 3,244,131 to 3,244,562 _RS15485 BWI76_RS15490: BWI76_RS15490 - mannitol dehydrogenase family protein, at 3,244,841 to 3,246,304 _RS15490 Position (kb) 3244 3245Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 3243.241 kb on - strandat 3243.323 kb on + strandat 3243.475 kb on + strand, within BWI76_RS15480at 3243.631 kb on - strand, within BWI76_RS15480at 3243.646 kb on - strand, within BWI76_RS15480at 3243.752 kb on + strand, within BWI76_RS15480at 3243.776 kb on - strand, within BWI76_RS15480at 3243.846 kb on + strand, within BWI76_RS15480at 3243.846 kb on + strand, within BWI76_RS15480at 3243.895 kb on + strand, within BWI76_RS15480at 3243.895 kb on + strand, within BWI76_RS15480at 3243.919 kb on - strand, within BWI76_RS15480at 3243.926 kb on - strand, within BWI76_RS15480at 3243.976 kb on + strand, within BWI76_RS15480at 3243.978 kb on - strand, within BWI76_RS15480at 3243.985 kb on - strand, within BWI76_RS15480at 3243.985 kb on - strand, within BWI76_RS15480at 3244.003 kb on + strand, within BWI76_RS15480at 3244.011 kb on - strandat 3244.011 kb on - strandat 3244.011 kb on - strandat 3244.011 kb on - strandat 3244.094 kb on + strandat 3244.124 kb on - strandat 3244.212 kb on - strand, within BWI76_RS15485at 3244.276 kb on - strand, within BWI76_RS15485at 3244.320 kb on - strand, within BWI76_RS15485at 3244.332 kb on - strand, within BWI76_RS15485at 3244.338 kb on + strand, within BWI76_RS15485at 3244.346 kb on - strand, within BWI76_RS15485at 3244.346 kb on - strand, within BWI76_RS15485at 3244.357 kb on + strand, within BWI76_RS15485at 3244.382 kb on - strand, within BWI76_RS15485at 3244.382 kb on - strand, within BWI76_RS15485at 3244.439 kb on + strand, within BWI76_RS15485at 3244.452 kb on - strand, within BWI76_RS15485at 3244.452 kb on - strand, within BWI76_RS15485at 3244.452 kb on - strand, within BWI76_RS15485at 3244.452 kb on - strand, within BWI76_RS15485at 3244.494 kb on + strand, within BWI76_RS15485at 3244.520 kb on - strandat 3244.540 kb on - strandat 3244.540 kb on - strandat 3244.853 kb on + strandat 3244.853 kb on + strandat 3244.854 kb on + strandat 3244.861 kb on - strandat 3244.862 kb on - strandat 3244.873 kb on + strandat 3244.873 kb on + strandat 3244.873 kb on + strandat 3244.873 kb on + strandat 3244.881 kb on - strandat 3244.881 kb on - strandat 3244.881 kb on - strandat 3244.913 kb on - strandat 3244.935 kb on + strandat 3244.940 kb on + strandat 3244.945 kb on + strandat 3245.009 kb on + strand, within BWI76_RS15490at 3245.047 kb on - strand, within BWI76_RS15490at 3245.065 kb on + strand, within BWI76_RS15490at 3245.440 kb on + strand, within BWI76_RS15490

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,243,241 - -0.2
3,243,323 + -0.8
3,243,475 + BWI76_RS15480 0.12 +0.6
3,243,631 - BWI76_RS15480 0.35 -3.9
3,243,646 - BWI76_RS15480 0.37 +0.8
3,243,752 + BWI76_RS15480 0.52 -1.9
3,243,776 - BWI76_RS15480 0.56 +0.9
3,243,846 + BWI76_RS15480 0.66 -2.9
3,243,846 + BWI76_RS15480 0.66 +0.3
3,243,895 + BWI76_RS15480 0.73 -2.1
3,243,895 + BWI76_RS15480 0.73 -0.1
3,243,919 - BWI76_RS15480 0.77 +0.2
3,243,926 - BWI76_RS15480 0.78 -0.1
3,243,976 + BWI76_RS15480 0.85 +1.6
3,243,978 - BWI76_RS15480 0.85 -2.8
3,243,985 - BWI76_RS15480 0.86 -1.1
3,243,985 - BWI76_RS15480 0.86 +0.9
3,244,003 + BWI76_RS15480 0.89 +0.9
3,244,011 - -1.9
3,244,011 - +0.9
3,244,011 - +0.9
3,244,011 - +0.7
3,244,094 + -1.0
3,244,124 - +0.5
3,244,212 - BWI76_RS15485 0.19 -1.2
3,244,276 - BWI76_RS15485 0.34 -1.7
3,244,320 - BWI76_RS15485 0.44 -1.9
3,244,332 - BWI76_RS15485 0.47 -0.6
3,244,338 + BWI76_RS15485 0.48 -1.0
3,244,346 - BWI76_RS15485 0.50 -1.5
3,244,346 - BWI76_RS15485 0.50 -2.2
3,244,357 + BWI76_RS15485 0.52 -1.1
3,244,382 - BWI76_RS15485 0.58 -0.6
3,244,382 - BWI76_RS15485 0.58 -0.4
3,244,439 + BWI76_RS15485 0.71 +0.9
3,244,452 - BWI76_RS15485 0.74 -3.4
3,244,452 - BWI76_RS15485 0.74 -0.1
3,244,452 - BWI76_RS15485 0.74 -0.1
3,244,452 - BWI76_RS15485 0.74 -1.8
3,244,494 + BWI76_RS15485 0.84 -0.1
3,244,520 - +0.3
3,244,540 - -0.7
3,244,540 - -0.1
3,244,853 + -0.1
3,244,853 + +3.5
3,244,854 + -3.7
3,244,861 - -0.2
3,244,862 - -2.5
3,244,873 + -3.5
3,244,873 + -1.5
3,244,873 + +0.9
3,244,873 + -2.8
3,244,881 - +0.3
3,244,881 - +0.7
3,244,881 - -0.2
3,244,913 - +0.9
3,244,935 + +1.3
3,244,940 + -1.9
3,244,945 + +0.3
3,245,009 + BWI76_RS15490 0.11 -0.5
3,245,047 - BWI76_RS15490 0.14 +1.1
3,245,065 + BWI76_RS15490 0.15 -2.9
3,245,440 + BWI76_RS15490 0.41 -2.9

Or see this region's nucleotide sequence