Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15475

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15470 and BWI76_RS15475 are separated by 172 nucleotidesBWI76_RS15475 and BWI76_RS15480 are separated by 90 nucleotidesBWI76_RS15480 and BWI76_RS15485 are separated by 51 nucleotides BWI76_RS15470: BWI76_RS15470 - dipeptidyl carboxypeptidase II, at 3,240,338 to 3,242,380 _RS15470 BWI76_RS15475: BWI76_RS15475 - NAD(P)-dependent oxidoreductase, at 3,242,553 to 3,243,302 _RS15475 BWI76_RS15480: BWI76_RS15480 - GntR family transcriptional regulator, at 3,243,393 to 3,244,079 _RS15480 BWI76_RS15485: BWI76_RS15485 - putative universal stress protein G, at 3,244,131 to 3,244,562 _RS15485 Position (kb) 3242 3243 3244Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3241.674 kb on - strand, within BWI76_RS15470at 3242.421 kb on + strandat 3242.517 kb on + strandat 3242.693 kb on + strand, within BWI76_RS15475at 3242.693 kb on + strand, within BWI76_RS15475at 3242.693 kb on + strand, within BWI76_RS15475at 3242.701 kb on - strand, within BWI76_RS15475at 3242.703 kb on + strand, within BWI76_RS15475at 3242.844 kb on - strand, within BWI76_RS15475at 3242.846 kb on - strand, within BWI76_RS15475at 3242.914 kb on - strand, within BWI76_RS15475at 3242.915 kb on + strand, within BWI76_RS15475at 3243.241 kb on - strandat 3243.323 kb on + strandat 3243.475 kb on + strand, within BWI76_RS15480at 3243.631 kb on - strand, within BWI76_RS15480at 3243.646 kb on - strand, within BWI76_RS15480at 3243.752 kb on + strand, within BWI76_RS15480at 3243.776 kb on - strand, within BWI76_RS15480at 3243.846 kb on + strand, within BWI76_RS15480at 3243.846 kb on + strand, within BWI76_RS15480at 3243.895 kb on + strand, within BWI76_RS15480at 3243.895 kb on + strand, within BWI76_RS15480at 3243.919 kb on - strand, within BWI76_RS15480at 3243.926 kb on - strand, within BWI76_RS15480at 3243.976 kb on + strand, within BWI76_RS15480at 3243.978 kb on - strand, within BWI76_RS15480at 3243.985 kb on - strand, within BWI76_RS15480at 3243.985 kb on - strand, within BWI76_RS15480at 3244.003 kb on + strand, within BWI76_RS15480at 3244.011 kb on - strandat 3244.011 kb on - strandat 3244.011 kb on - strandat 3244.011 kb on - strandat 3244.094 kb on + strandat 3244.124 kb on - strandat 3244.212 kb on - strand, within BWI76_RS15485at 3244.276 kb on - strand, within BWI76_RS15485

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,241,674 - BWI76_RS15470 0.65 +0.0
3,242,421 + -0.2
3,242,517 + +1.3
3,242,693 + BWI76_RS15475 0.19 -0.1
3,242,693 + BWI76_RS15475 0.19 +1.3
3,242,693 + BWI76_RS15475 0.19 +0.5
3,242,701 - BWI76_RS15475 0.20 -0.8
3,242,703 + BWI76_RS15475 0.20 -2.5
3,242,844 - BWI76_RS15475 0.39 +0.2
3,242,846 - BWI76_RS15475 0.39 +0.5
3,242,914 - BWI76_RS15475 0.48 +0.8
3,242,915 + BWI76_RS15475 0.48 -1.5
3,243,241 - -0.2
3,243,323 + -0.8
3,243,475 + BWI76_RS15480 0.12 +0.6
3,243,631 - BWI76_RS15480 0.35 -3.9
3,243,646 - BWI76_RS15480 0.37 +0.8
3,243,752 + BWI76_RS15480 0.52 -1.9
3,243,776 - BWI76_RS15480 0.56 +0.9
3,243,846 + BWI76_RS15480 0.66 -2.9
3,243,846 + BWI76_RS15480 0.66 +0.3
3,243,895 + BWI76_RS15480 0.73 -2.1
3,243,895 + BWI76_RS15480 0.73 -0.1
3,243,919 - BWI76_RS15480 0.77 +0.2
3,243,926 - BWI76_RS15480 0.78 -0.1
3,243,976 + BWI76_RS15480 0.85 +1.6
3,243,978 - BWI76_RS15480 0.85 -2.8
3,243,985 - BWI76_RS15480 0.86 -1.1
3,243,985 - BWI76_RS15480 0.86 +0.9
3,244,003 + BWI76_RS15480 0.89 +0.9
3,244,011 - -1.9
3,244,011 - +0.9
3,244,011 - +0.9
3,244,011 - +0.7
3,244,094 + -1.0
3,244,124 - +0.5
3,244,212 - BWI76_RS15485 0.19 -1.2
3,244,276 - BWI76_RS15485 0.34 -1.7

Or see this region's nucleotide sequence