Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS15190

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS15180 and BWI76_RS15185 are separated by 111 nucleotidesBWI76_RS15185 and BWI76_RS15190 are separated by 90 nucleotidesBWI76_RS15190 and BWI76_RS15195 are separated by 107 nucleotidesBWI76_RS15195 and BWI76_RS15200 overlap by 1 nucleotides BWI76_RS15180: BWI76_RS15180 - altronate oxidoreductase, at 3,181,934 to 3,183,385 _RS15180 BWI76_RS15185: BWI76_RS15185 - N-acetyltransferase, at 3,183,497 to 3,184,027 _RS15185 BWI76_RS15190: BWI76_RS15190 - GGDEF domain-containing protein, at 3,184,118 to 3,185,530 _RS15190 BWI76_RS15195: BWI76_RS15195 - DUF4186 domain-containing protein, at 3,185,638 to 3,186,012 _RS15195 BWI76_RS15200: BWI76_RS15200 - glutaminase, at 3,186,012 to 3,186,938 _RS15200 Position (kb) 3184 3185 3186Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3183.141 kb on + strandat 3183.141 kb on + strand, within BWI76_RS15180at 3183.345 kb on - strandat 3183.648 kb on + strand, within BWI76_RS15185at 3183.910 kb on + strand, within BWI76_RS15185at 3184.420 kb on + strand, within BWI76_RS15190at 3184.503 kb on - strand, within BWI76_RS15190at 3184.511 kb on + strand, within BWI76_RS15190at 3184.607 kb on + strand, within BWI76_RS15190at 3184.979 kb on - strand, within BWI76_RS15190at 3185.286 kb on - strand, within BWI76_RS15190at 3185.294 kb on - strand, within BWI76_RS15190at 3185.446 kb on + strandat 3185.446 kb on + strandat 3185.543 kb on + strandat 3185.710 kb on - strand, within BWI76_RS15195at 3185.881 kb on - strand, within BWI76_RS15195at 3186.093 kb on - strandat 3186.326 kb on + strand, within BWI76_RS15200

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,183,141 + +0.9
3,183,141 + BWI76_RS15180 0.83 +1.4
3,183,345 - -1.5
3,183,648 + BWI76_RS15185 0.28 -1.1
3,183,910 + BWI76_RS15185 0.78 +0.3
3,184,420 + BWI76_RS15190 0.21 +2.0
3,184,503 - BWI76_RS15190 0.27 -0.6
3,184,511 + BWI76_RS15190 0.28 +1.2
3,184,607 + BWI76_RS15190 0.35 +0.5
3,184,979 - BWI76_RS15190 0.61 +0.7
3,185,286 - BWI76_RS15190 0.83 +1.6
3,185,294 - BWI76_RS15190 0.83 -1.9
3,185,446 + +1.2
3,185,446 + +1.0
3,185,543 + +0.9
3,185,710 - BWI76_RS15195 0.19 +0.1
3,185,881 - BWI76_RS15195 0.65 -2.8
3,186,093 - +0.5
3,186,326 + BWI76_RS15200 0.34 +0.5

Or see this region's nucleotide sequence