Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15070 and BWI76_RS15075 are separated by 65 nucleotides BWI76_RS15075 and BWI76_RS15080 are separated by 2 nucleotides BWI76_RS15080 and BWI76_RS15085 are separated by 458 nucleotides
BWI76_RS15070: BWI76_RS15070 - non-ribosomal peptide synthetase, at 3,153,366 to 3,157,736
_RS15070
BWI76_RS15075: BWI76_RS15075 - non-ribosomal peptide synthase, at 3,157,802 to 3,158,032
_RS15075
BWI76_RS15080: BWI76_RS15080 - hypothetical protein, at 3,158,035 to 3,159,555
_RS15080
BWI76_RS15085: BWI76_RS15085 - 3-deoxy-D-arabinose-heptulosonic-7-phosphate synthase, at 3,160,014 to 3,161,183
_RS15085
Position (kb)
3158
3159
3160 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3157.182 kb on + strand, within BWI76_RS15070 at 3157.252 kb on - strand, within BWI76_RS15070 at 3157.280 kb on + strand, within BWI76_RS15070 at 3157.313 kb on + strand at 3157.313 kb on + strand at 3157.313 kb on + strand at 3157.321 kb on - strand at 3157.321 kb on - strand at 3157.347 kb on + strand at 3157.355 kb on - strand at 3157.387 kb on - strand at 3157.402 kb on - strand at 3157.547 kb on - strand at 3157.767 kb on + strand at 3157.819 kb on + strand at 3157.827 kb on + strand at 3157.827 kb on + strand, within BWI76_RS15075 at 3157.827 kb on + strand, within BWI76_RS15075 at 3157.827 kb on + strand, within BWI76_RS15075 at 3157.827 kb on - strand, within BWI76_RS15075 at 3157.835 kb on - strand, within BWI76_RS15075 at 3157.835 kb on - strand, within BWI76_RS15075 at 3157.835 kb on - strand, within BWI76_RS15075 at 3158.009 kb on - strand, within BWI76_RS15075 at 3158.012 kb on + strand at 3158.033 kb on + strand at 3158.033 kb on + strand at 3158.093 kb on + strand at 3158.154 kb on - strand at 3158.197 kb on - strand, within BWI76_RS15080 at 3158.268 kb on - strand, within BWI76_RS15080 at 3158.270 kb on + strand, within BWI76_RS15080 at 3158.366 kb on + strand, within BWI76_RS15080 at 3158.371 kb on + strand, within BWI76_RS15080 at 3158.471 kb on + strand, within BWI76_RS15080 at 3158.476 kb on + strand, within BWI76_RS15080 at 3158.476 kb on + strand, within BWI76_RS15080 at 3158.535 kb on - strand at 3158.567 kb on + strand, within BWI76_RS15080 at 3158.575 kb on - strand, within BWI76_RS15080 at 3158.590 kb on - strand, within BWI76_RS15080 at 3158.599 kb on - strand, within BWI76_RS15080 at 3158.600 kb on - strand, within BWI76_RS15080 at 3158.638 kb on - strand, within BWI76_RS15080 at 3158.676 kb on - strand, within BWI76_RS15080 at 3158.754 kb on - strand, within BWI76_RS15080 at 3158.762 kb on + strand, within BWI76_RS15080 at 3158.816 kb on - strand, within BWI76_RS15080 at 3158.901 kb on + strand, within BWI76_RS15080 at 3158.975 kb on + strand at 3158.995 kb on + strand, within BWI76_RS15080 at 3158.998 kb on - strand, within BWI76_RS15080 at 3159.080 kb on - strand, within BWI76_RS15080 at 3159.080 kb on - strand, within BWI76_RS15080 at 3159.080 kb on - strand, within BWI76_RS15080 at 3159.091 kb on - strand, within BWI76_RS15080 at 3159.177 kb on - strand, within BWI76_RS15080 at 3159.183 kb on - strand, within BWI76_RS15080 at 3159.188 kb on - strand, within BWI76_RS15080 at 3159.230 kb on - strand, within BWI76_RS15080 at 3159.250 kb on - strand, within BWI76_RS15080 at 3159.250 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.272 kb on - strand, within BWI76_RS15080 at 3159.274 kb on + strand, within BWI76_RS15080 at 3159.289 kb on + strand, within BWI76_RS15080 at 3159.289 kb on + strand, within BWI76_RS15080 at 3159.289 kb on + strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.297 kb on - strand, within BWI76_RS15080 at 3159.299 kb on - strand, within BWI76_RS15080 at 3159.299 kb on - strand, within BWI76_RS15080 at 3159.299 kb on - strand, within BWI76_RS15080 at 3159.299 kb on - strand, within BWI76_RS15080 at 3159.313 kb on - strand, within BWI76_RS15080 at 3159.344 kb on - strand, within BWI76_RS15080 at 3159.409 kb on - strand at 3159.410 kb on - strand at 3159.411 kb on + strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.419 kb on - strand at 3159.421 kb on - strand at 3159.429 kb on - strand at 3159.429 kb on - strand at 3159.476 kb on - strand at 3159.476 kb on - strand at 3159.476 kb on - strand at 3159.529 kb on - strand at 3159.529 kb on - strand at 3159.543 kb on - strand at 3159.543 kb on - strand at 3159.570 kb on - strand at 3159.570 kb on - strand at 3159.590 kb on + strand at 3159.598 kb on - strand at 3159.598 kb on - strand at 3159.598 kb on - strand at 3159.648 kb on - strand at 3159.654 kb on - strand at 3159.686 kb on + strand at 3159.714 kb on - strand at 3159.717 kb on + strand at 3159.735 kb on - strand at 3159.913 kb on + strand at 3159.913 kb on + strand at 3159.914 kb on + strand at 3159.931 kb on - strand at 3159.939 kb on + strand at 3160.045 kb on - strand at 3160.053 kb on - strand at 3160.058 kb on - strand at 3160.058 kb on - strand at 3160.063 kb on - strand at 3160.070 kb on + strand at 3160.070 kb on + strand at 3160.075 kb on + strand at 3160.077 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.078 kb on - strand at 3160.083 kb on - strand at 3160.083 kb on - strand at 3160.083 kb on - strand at 3160.083 kb on - strand at 3160.085 kb on + strand at 3160.085 kb on + strand at 3160.093 kb on - strand at 3160.095 kb on + strand at 3160.103 kb on - strand at 3160.103 kb on - strand at 3160.107 kb on + strand at 3160.115 kb on - strand at 3160.160 kb on - strand, within BWI76_RS15085 at 3160.176 kb on - strand, within BWI76_RS15085 at 3160.222 kb on + strand, within BWI76_RS15085 at 3160.230 kb on - strand, within BWI76_RS15085 at 3160.258 kb on + strand, within BWI76_RS15085 at 3160.296 kb on - strand at 3160.358 kb on + strand, within BWI76_RS15085 at 3160.373 kb on + strand, within BWI76_RS15085 at 3160.386 kb on - strand, within BWI76_RS15085 at 3160.387 kb on - strand, within BWI76_RS15085 at 3160.391 kb on - strand, within BWI76_RS15085 at 3160.463 kb on + strand, within BWI76_RS15085 at 3160.477 kb on - strand, within BWI76_RS15085 at 3160.552 kb on + strand, within BWI76_RS15085
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,157,182 + BWI76_RS15070 0.87 +1.3 3,157,252 - BWI76_RS15070 0.89 -0.1 3,157,280 + BWI76_RS15070 0.90 -0.3 3,157,313 + -0.9 3,157,313 + +0.8 3,157,313 + -0.1 3,157,321 - -2.3 3,157,321 - +0.8 3,157,347 + -0.7 3,157,355 - +1.2 3,157,387 - +1.2 3,157,402 - +1.5 3,157,547 - +0.1 3,157,767 + -0.5 3,157,819 + -0.7 3,157,827 + -0.7 3,157,827 + BWI76_RS15075 0.11 -2.8 3,157,827 + BWI76_RS15075 0.11 -2.3 3,157,827 + BWI76_RS15075 0.11 -0.5 3,157,827 - BWI76_RS15075 0.11 +1.2 3,157,835 - BWI76_RS15075 0.14 +2.2 3,157,835 - BWI76_RS15075 0.14 +0.5 3,157,835 - BWI76_RS15075 0.14 +1.6 3,158,009 - BWI76_RS15075 0.90 +0.5 3,158,012 + +0.5 3,158,033 + +0.7 3,158,033 + +0.1 3,158,093 + +0.7 3,158,154 - -0.1 3,158,197 - BWI76_RS15080 0.11 +0.6 3,158,268 - BWI76_RS15080 0.15 -1.9 3,158,270 + BWI76_RS15080 0.15 +0.9 3,158,366 + BWI76_RS15080 0.22 +0.9 3,158,371 + BWI76_RS15080 0.22 +0.6 3,158,471 + BWI76_RS15080 0.29 -0.0 3,158,476 + BWI76_RS15080 0.29 +0.9 3,158,476 + BWI76_RS15080 0.29 +1.0 3,158,535 - -0.7 3,158,567 + BWI76_RS15080 0.35 -3.6 3,158,575 - BWI76_RS15080 0.36 +1.1 3,158,590 - BWI76_RS15080 0.36 +0.9 3,158,599 - BWI76_RS15080 0.37 +1.2 3,158,600 - BWI76_RS15080 0.37 +1.1 3,158,638 - BWI76_RS15080 0.40 +0.3 3,158,676 - BWI76_RS15080 0.42 +0.6 3,158,754 - BWI76_RS15080 0.47 +0.9 3,158,762 + BWI76_RS15080 0.48 -0.2 3,158,816 - BWI76_RS15080 0.51 +1.1 3,158,901 + BWI76_RS15080 0.57 +0.7 3,158,975 + +1.5 3,158,995 + BWI76_RS15080 0.63 -0.7 3,158,998 - BWI76_RS15080 0.63 +1.4 3,159,080 - BWI76_RS15080 0.69 -1.7 3,159,080 - BWI76_RS15080 0.69 +0.8 3,159,080 - BWI76_RS15080 0.69 +0.8 3,159,091 - BWI76_RS15080 0.69 -1.9 3,159,177 - BWI76_RS15080 0.75 +1.1 3,159,183 - BWI76_RS15080 0.75 +0.9 3,159,188 - BWI76_RS15080 0.76 +0.0 3,159,230 - BWI76_RS15080 0.79 +0.4 3,159,250 - BWI76_RS15080 0.80 +0.9 3,159,250 - BWI76_RS15080 0.80 +0.5 3,159,272 - BWI76_RS15080 0.81 +0.3 3,159,272 - BWI76_RS15080 0.81 +0.5 3,159,272 - BWI76_RS15080 0.81 +1.2 3,159,272 - BWI76_RS15080 0.81 +0.9 3,159,272 - BWI76_RS15080 0.81 -0.7 3,159,272 - BWI76_RS15080 0.81 +0.6 3,159,272 - BWI76_RS15080 0.81 +0.9 3,159,274 + BWI76_RS15080 0.81 +1.9 3,159,289 + BWI76_RS15080 0.82 +1.3 3,159,289 + BWI76_RS15080 0.82 +1.7 3,159,289 + BWI76_RS15080 0.82 -0.7 3,159,297 - BWI76_RS15080 0.83 +1.0 3,159,297 - BWI76_RS15080 0.83 +1.7 3,159,297 - BWI76_RS15080 0.83 -1.7 3,159,297 - BWI76_RS15080 0.83 +0.9 3,159,297 - BWI76_RS15080 0.83 +0.9 3,159,297 - BWI76_RS15080 0.83 +1.2 3,159,297 - BWI76_RS15080 0.83 +4.0 3,159,297 - BWI76_RS15080 0.83 +1.2 3,159,297 - BWI76_RS15080 0.83 +0.8 3,159,297 - BWI76_RS15080 0.83 +0.8 3,159,297 - BWI76_RS15080 0.83 +1.3 3,159,297 - BWI76_RS15080 0.83 +0.8 3,159,297 - BWI76_RS15080 0.83 +1.0 3,159,297 - BWI76_RS15080 0.83 +0.7 3,159,297 - BWI76_RS15080 0.83 +1.7 3,159,297 - BWI76_RS15080 0.83 +0.7 3,159,297 - BWI76_RS15080 0.83 +0.3 3,159,297 - BWI76_RS15080 0.83 +0.9 3,159,297 - BWI76_RS15080 0.83 +1.0 3,159,297 - BWI76_RS15080 0.83 +1.5 3,159,297 - BWI76_RS15080 0.83 +0.7 3,159,297 - BWI76_RS15080 0.83 +1.1 3,159,299 - BWI76_RS15080 0.83 +1.4 3,159,299 - BWI76_RS15080 0.83 +1.1 3,159,299 - BWI76_RS15080 0.83 +1.1 3,159,299 - BWI76_RS15080 0.83 +2.5 3,159,313 - BWI76_RS15080 0.84 -0.0 3,159,344 - BWI76_RS15080 0.86 +1.2 3,159,409 - +1.7 3,159,410 - +2.1 3,159,411 + +0.4 3,159,419 - -3.9 3,159,419 - +0.6 3,159,419 - +0.6 3,159,419 - -0.5 3,159,419 - -0.9 3,159,419 - -1.5 3,159,419 - +0.9 3,159,419 - +0.7 3,159,419 - +1.3 3,159,419 - +0.4 3,159,421 - +0.4 3,159,429 - +0.9 3,159,429 - +0.4 3,159,476 - -0.8 3,159,476 - +1.1 3,159,476 - +0.8 3,159,529 - +0.9 3,159,529 - +0.9 3,159,543 - -1.7 3,159,543 - +1.6 3,159,570 - -1.4 3,159,570 - +1.4 3,159,590 + +1.0 3,159,598 - +1.4 3,159,598 - -0.5 3,159,598 - +0.6 3,159,648 - -2.8 3,159,654 - +0.4 3,159,686 + -0.5 3,159,714 - +0.9 3,159,717 + +0.9 3,159,735 - -1.0 3,159,913 + +0.6 3,159,913 + +1.1 3,159,914 + +0.9 3,159,931 - -0.9 3,159,939 + +0.6 3,160,045 - -1.1 3,160,053 - -0.4 3,160,058 - -2.5 3,160,058 - -3.0 3,160,063 - +1.3 3,160,070 + -0.3 3,160,070 + +1.2 3,160,075 + +0.5 3,160,077 - +1.0 3,160,078 - +4.2 3,160,078 - +0.9 3,160,078 - +0.6 3,160,078 - -1.7 3,160,078 - +0.9 3,160,078 - +0.6 3,160,078 - +1.4 3,160,078 - +2.0 3,160,083 - +1.0 3,160,083 - -1.5 3,160,083 - +0.9 3,160,083 - -0.4 3,160,085 + +1.5 3,160,085 + +0.4 3,160,093 - -2.3 3,160,095 + -2.1 3,160,103 - +0.7 3,160,103 - +0.5 3,160,107 + -0.6 3,160,115 - -0.7 3,160,160 - BWI76_RS15085 0.12 -2.1 3,160,176 - BWI76_RS15085 0.14 +0.6 3,160,222 + BWI76_RS15085 0.18 +0.9 3,160,230 - BWI76_RS15085 0.18 +0.4 3,160,258 + BWI76_RS15085 0.21 +0.9 3,160,296 - +3.5 3,160,358 + BWI76_RS15085 0.29 +0.9 3,160,373 + BWI76_RS15085 0.31 +2.0 3,160,386 - BWI76_RS15085 0.32 +0.6 3,160,387 - BWI76_RS15085 0.32 -0.6 3,160,391 - BWI76_RS15085 0.32 -0.2 3,160,463 + BWI76_RS15085 0.38 +1.7 3,160,477 - BWI76_RS15085 0.40 +1.0 3,160,552 + BWI76_RS15085 0.46 +1.3
Or see this region's nucleotide sequence