Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14960 and BWI76_RS14965 are separated by 108 nucleotides BWI76_RS14965 and BWI76_RS14970 overlap by 4 nucleotides BWI76_RS14970 and BWI76_RS14975 are separated by 12 nucleotides
BWI76_RS14960: BWI76_RS14960 - MFS transporter, at 3,135,812 to 3,137,011
_RS14960
BWI76_RS14965: BWI76_RS14965 - AraC family transcriptional regulator, at 3,137,120 to 3,138,076
_RS14965
BWI76_RS14970: BWI76_RS14970 - pyrroline-5-carboxylate reductase, at 3,138,073 to 3,138,861
_RS14970
BWI76_RS14975: BWI76_RS14975 - glyoxalase-like domain protein, at 3,138,874 to 3,139,629
_RS14975
Position (kb)
3137
3138
3139 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3136.243 kb on + strand, within BWI76_RS14960 at 3136.251 kb on - strand, within BWI76_RS14960 at 3136.251 kb on - strand, within BWI76_RS14960 at 3136.289 kb on - strand, within BWI76_RS14960 at 3136.381 kb on - strand, within BWI76_RS14960 at 3136.427 kb on + strand, within BWI76_RS14960 at 3136.434 kb on + strand, within BWI76_RS14960 at 3136.792 kb on + strand, within BWI76_RS14960 at 3136.844 kb on + strand, within BWI76_RS14960 at 3136.928 kb on - strand at 3137.141 kb on - strand at 3137.265 kb on + strand, within BWI76_RS14965 at 3137.467 kb on + strand, within BWI76_RS14965 at 3137.507 kb on - strand, within BWI76_RS14965 at 3137.932 kb on - strand, within BWI76_RS14965 at 3137.985 kb on - strand at 3138.616 kb on - strand, within BWI76_RS14970
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,136,243 + BWI76_RS14960 0.36 -2.1 3,136,251 - BWI76_RS14960 0.37 +0.9 3,136,251 - BWI76_RS14960 0.37 -2.1 3,136,289 - BWI76_RS14960 0.40 +0.2 3,136,381 - BWI76_RS14960 0.47 +0.8 3,136,427 + BWI76_RS14960 0.51 -0.2 3,136,434 + BWI76_RS14960 0.52 -1.1 3,136,792 + BWI76_RS14960 0.82 +1.2 3,136,844 + BWI76_RS14960 0.86 -0.2 3,136,928 - +1.2 3,137,141 - +0.2 3,137,265 + BWI76_RS14965 0.15 +1.4 3,137,467 + BWI76_RS14965 0.36 +0.3 3,137,507 - BWI76_RS14965 0.40 +2.4 3,137,932 - BWI76_RS14965 0.85 +0.4 3,137,985 - +0.0 3,138,616 - BWI76_RS14970 0.69 +0.4
Or see this region's nucleotide sequence