Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14825 and BWI76_RS14830 overlap by 1 nucleotides BWI76_RS14830 and BWI76_RS14835 are separated by 133 nucleotides BWI76_RS14835 and BWI76_RS14840 are separated by 180 nucleotides BWI76_RS14840 and BWI76_RS14845 are separated by 208 nucleotides
BWI76_RS14825: BWI76_RS14825 - dienelactone hydrolase family protein, at 3,106,733 to 3,107,332
_RS14825
BWI76_RS14830: BWI76_RS14830 - hypothetical protein, at 3,107,332 to 3,107,904
_RS14830
BWI76_RS14835: BWI76_RS14835 - hypothetical protein, at 3,108,038 to 3,108,430
_RS14835
BWI76_RS14840: BWI76_RS14840 - hypothetical protein, at 3,108,611 to 3,109,213
_RS14840
BWI76_RS14845: BWI76_RS14845 - hypothetical protein, at 3,109,422 to 3,110,048
_RS14845
Position (kb)
3108
3109 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3107.604 kb on - strand, within BWI76_RS14830 at 3107.727 kb on - strand, within BWI76_RS14830 at 3107.735 kb on + strand, within BWI76_RS14830 at 3107.821 kb on + strand, within BWI76_RS14830 at 3108.022 kb on + strand at 3108.130 kb on - strand, within BWI76_RS14835 at 3108.356 kb on + strand, within BWI76_RS14835 at 3108.425 kb on + strand at 3108.594 kb on - strand at 3108.726 kb on - strand, within BWI76_RS14840 at 3108.847 kb on - strand, within BWI76_RS14840 at 3109.135 kb on + strand, within BWI76_RS14840 at 3109.175 kb on - strand at 3109.240 kb on + strand at 3109.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,107,604 - BWI76_RS14830 0.47 -1.1 3,107,727 - BWI76_RS14830 0.69 +1.0 3,107,735 + BWI76_RS14830 0.70 -0.0 3,107,821 + BWI76_RS14830 0.85 +0.2 3,108,022 + -3.3 3,108,130 - BWI76_RS14835 0.23 +1.1 3,108,356 + BWI76_RS14835 0.81 +1.9 3,108,425 + +0.5 3,108,594 - +0.7 3,108,726 - BWI76_RS14840 0.19 +0.1 3,108,847 - BWI76_RS14840 0.39 +0.2 3,109,135 + BWI76_RS14840 0.87 -0.1 3,109,175 - +0.8 3,109,240 + -0.2 3,109,361 - +0.4
Or see this region's nucleotide sequence