Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14825

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14820 and BWI76_RS14825 are separated by 210 nucleotidesBWI76_RS14825 and BWI76_RS14830 overlap by 1 nucleotidesBWI76_RS14830 and BWI76_RS14835 are separated by 133 nucleotides BWI76_RS14820: BWI76_RS14820 - LysR family transcriptional regulator, at 3,105,614 to 3,106,522 _RS14820 BWI76_RS14825: BWI76_RS14825 - dienelactone hydrolase family protein, at 3,106,733 to 3,107,332 _RS14825 BWI76_RS14830: BWI76_RS14830 - hypothetical protein, at 3,107,332 to 3,107,904 _RS14830 BWI76_RS14835: BWI76_RS14835 - hypothetical protein, at 3,108,038 to 3,108,430 _RS14835 Position (kb) 3106 3107 3108Strain fitness (log2 ratio) -3 -2 -1 0 1at 3106.369 kb on + strand, within BWI76_RS14820at 3106.660 kb on - strandat 3106.674 kb on - strandat 3106.846 kb on - strand, within BWI76_RS14825at 3106.983 kb on + strand, within BWI76_RS14825at 3107.604 kb on - strand, within BWI76_RS14830at 3107.727 kb on - strand, within BWI76_RS14830at 3107.735 kb on + strand, within BWI76_RS14830at 3107.821 kb on + strand, within BWI76_RS14830at 3108.022 kb on + strandat 3108.130 kb on - strand, within BWI76_RS14835

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,106,369 + BWI76_RS14820 0.83 +0.2
3,106,660 - -0.8
3,106,674 - +0.1
3,106,846 - BWI76_RS14825 0.19 +1.2
3,106,983 + BWI76_RS14825 0.42 +0.8
3,107,604 - BWI76_RS14830 0.47 -1.1
3,107,727 - BWI76_RS14830 0.69 +1.0
3,107,735 + BWI76_RS14830 0.70 -0.0
3,107,821 + BWI76_RS14830 0.85 +0.2
3,108,022 + -3.3
3,108,130 - BWI76_RS14835 0.23 +1.1

Or see this region's nucleotide sequence