Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14820 and BWI76_RS14825 are separated by 210 nucleotides BWI76_RS14825 and BWI76_RS14830 overlap by 1 nucleotides BWI76_RS14830 and BWI76_RS14835 are separated by 133 nucleotides
BWI76_RS14820: BWI76_RS14820 - LysR family transcriptional regulator, at 3,105,614 to 3,106,522
_RS14820
BWI76_RS14825: BWI76_RS14825 - dienelactone hydrolase family protein, at 3,106,733 to 3,107,332
_RS14825
BWI76_RS14830: BWI76_RS14830 - hypothetical protein, at 3,107,332 to 3,107,904
_RS14830
BWI76_RS14835: BWI76_RS14835 - hypothetical protein, at 3,108,038 to 3,108,430
_RS14835
Position (kb)
3106
3107
3108 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3106.369 kb on + strand, within BWI76_RS14820 at 3106.660 kb on - strand at 3106.674 kb on - strand at 3106.846 kb on - strand, within BWI76_RS14825 at 3106.983 kb on + strand, within BWI76_RS14825 at 3107.604 kb on - strand, within BWI76_RS14830 at 3107.727 kb on - strand, within BWI76_RS14830 at 3107.735 kb on + strand, within BWI76_RS14830 at 3107.821 kb on + strand, within BWI76_RS14830 at 3108.022 kb on + strand at 3108.130 kb on - strand, within BWI76_RS14835
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,106,369 + BWI76_RS14820 0.83 +0.2 3,106,660 - -0.8 3,106,674 - +0.1 3,106,846 - BWI76_RS14825 0.19 +1.2 3,106,983 + BWI76_RS14825 0.42 +0.8 3,107,604 - BWI76_RS14830 0.47 -1.1 3,107,727 - BWI76_RS14830 0.69 +1.0 3,107,735 + BWI76_RS14830 0.70 -0.0 3,107,821 + BWI76_RS14830 0.85 +0.2 3,108,022 + -3.3 3,108,130 - BWI76_RS14835 0.23 +1.1
Or see this region's nucleotide sequence