Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14535 and BWI76_RS14540 overlap by 4 nucleotides BWI76_RS14540 and BWI76_RS14545 overlap by 4 nucleotides BWI76_RS14545 and BWI76_RS14550 are separated by 3 nucleotides
BWI76_RS14535: BWI76_RS14535 - polyketide synthase, at 3,042,203 to 3,051,679
_RS14535
BWI76_RS14540: BWI76_RS14540 - yersiniabactin biosynthetic protein YbtU, at 3,051,676 to 3,052,773
_RS14540
BWI76_RS14545: BWI76_RS14545 - thioesterase, at 3,052,770 to 3,053,573
_RS14545
BWI76_RS14550: BWI76_RS14550 - 2,3-dihydroxybenzoate-AMP ligase, at 3,053,577 to 3,055,154
_RS14550
Position (kb)
3051
3052
3053 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3050.710 kb on + strand, within BWI76_RS14535 at 3050.788 kb on + strand at 3050.838 kb on + strand at 3050.875 kb on - strand at 3051.608 kb on + strand at 3051.616 kb on - strand at 3051.749 kb on + strand at 3051.848 kb on + strand, within BWI76_RS14540 at 3052.055 kb on + strand, within BWI76_RS14540 at 3052.411 kb on + strand, within BWI76_RS14540 at 3052.830 kb on + strand at 3052.914 kb on + strand, within BWI76_RS14545 at 3052.948 kb on + strand, within BWI76_RS14545 at 3053.344 kb on + strand, within BWI76_RS14545 at 3053.384 kb on - strand, within BWI76_RS14545 at 3053.562 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 3,050,710 + BWI76_RS14535 0.90 -0.2 3,050,788 + +0.6 3,050,838 + -0.8 3,050,875 - -1.4 3,051,608 + +1.0 3,051,616 - +0.6 3,051,749 + +0.3 3,051,848 + BWI76_RS14540 0.16 +0.4 3,052,055 + BWI76_RS14540 0.35 +0.7 3,052,411 + BWI76_RS14540 0.67 +2.1 3,052,830 + -1.8 3,052,914 + BWI76_RS14545 0.18 +0.6 3,052,948 + BWI76_RS14545 0.22 +0.7 3,053,344 + BWI76_RS14545 0.71 +0.3 3,053,384 - BWI76_RS14545 0.76 +1.0 3,053,562 + -0.1
Or see this region's nucleotide sequence