Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14480

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14475 and BWI76_RS14480 are separated by 350 nucleotidesBWI76_RS14480 and BWI76_RS14485 overlap by 11 nucleotides BWI76_RS14475: BWI76_RS14475 - serine/threonine transporter, at 3,020,540 to 3,021,823 _RS14475 BWI76_RS14480: BWI76_RS14480 - hypothetical protein, at 3,022,174 to 3,023,424 _RS14480 BWI76_RS14485: BWI76_RS14485 - two-component sensor histidine kinase, at 3,023,414 to 3,025,423 _RS14485 Position (kb) 3022 3023 3024Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3021.249 kb on - strand, within BWI76_RS14475at 3021.257 kb on - strand, within BWI76_RS14475at 3021.257 kb on - strand, within BWI76_RS14475at 3021.259 kb on - strandat 3021.259 kb on - strand, within BWI76_RS14475at 3021.333 kb on + strand, within BWI76_RS14475at 3021.333 kb on + strand, within BWI76_RS14475at 3021.341 kb on - strand, within BWI76_RS14475at 3021.341 kb on - strand, within BWI76_RS14475at 3021.341 kb on - strand, within BWI76_RS14475at 3021.380 kb on - strand, within BWI76_RS14475at 3021.530 kb on + strand, within BWI76_RS14475at 3021.538 kb on - strand, within BWI76_RS14475at 3021.538 kb on - strand, within BWI76_RS14475at 3021.581 kb on - strand, within BWI76_RS14475at 3021.636 kb on - strand, within BWI76_RS14475at 3021.721 kb on - strandat 3021.726 kb on - strandat 3021.735 kb on + strandat 3021.770 kb on - strandat 3021.857 kb on - strandat 3021.930 kb on - strandat 3022.183 kb on + strandat 3022.189 kb on - strandat 3022.201 kb on - strandat 3022.201 kb on - strandat 3022.329 kb on - strand, within BWI76_RS14480at 3022.421 kb on - strand, within BWI76_RS14480at 3022.484 kb on + strand, within BWI76_RS14480at 3022.592 kb on + strand, within BWI76_RS14480at 3022.698 kb on + strand, within BWI76_RS14480at 3022.838 kb on - strand, within BWI76_RS14480at 3023.077 kb on - strand, within BWI76_RS14480at 3023.265 kb on + strand, within BWI76_RS14480at 3023.273 kb on - strand, within BWI76_RS14480at 3023.366 kb on - strandat 3023.536 kb on + strandat 3023.841 kb on - strand, within BWI76_RS14485at 3023.853 kb on - strand, within BWI76_RS14485at 3023.881 kb on - strand, within BWI76_RS14485at 3024.096 kb on + strand, within BWI76_RS14485at 3024.161 kb on - strand, within BWI76_RS14485at 3024.162 kb on + strand, within BWI76_RS14485at 3024.188 kb on + strand, within BWI76_RS14485at 3024.302 kb on - strand, within BWI76_RS14485

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,021,249 - BWI76_RS14475 0.55 -0.1
3,021,257 - BWI76_RS14475 0.56 -0.2
3,021,257 - BWI76_RS14475 0.56 -2.0
3,021,259 - +0.8
3,021,259 - BWI76_RS14475 0.56 +0.6
3,021,333 + BWI76_RS14475 0.62 +0.8
3,021,333 + BWI76_RS14475 0.62 -0.9
3,021,341 - BWI76_RS14475 0.62 -0.1
3,021,341 - BWI76_RS14475 0.62 +0.5
3,021,341 - BWI76_RS14475 0.62 -0.2
3,021,380 - BWI76_RS14475 0.65 +0.8
3,021,530 + BWI76_RS14475 0.77 -1.8
3,021,538 - BWI76_RS14475 0.78 -0.5
3,021,538 - BWI76_RS14475 0.78 -2.4
3,021,581 - BWI76_RS14475 0.81 -0.5
3,021,636 - BWI76_RS14475 0.85 -0.2
3,021,721 - +0.8
3,021,726 - -0.2
3,021,735 + +0.2
3,021,770 - -1.2
3,021,857 - -1.2
3,021,930 - -0.8
3,022,183 + +1.4
3,022,189 - +0.1
3,022,201 - +0.2
3,022,201 - +1.0
3,022,329 - BWI76_RS14480 0.12 -1.5
3,022,421 - BWI76_RS14480 0.20 +0.6
3,022,484 + BWI76_RS14480 0.25 -0.4
3,022,592 + BWI76_RS14480 0.33 -0.2
3,022,698 + BWI76_RS14480 0.42 -0.2
3,022,838 - BWI76_RS14480 0.53 +1.8
3,023,077 - BWI76_RS14480 0.72 +0.8
3,023,265 + BWI76_RS14480 0.87 +0.5
3,023,273 - BWI76_RS14480 0.88 -4.1
3,023,366 - +0.8
3,023,536 + -0.1
3,023,841 - BWI76_RS14485 0.21 -4.5
3,023,853 - BWI76_RS14485 0.22 +0.8
3,023,881 - BWI76_RS14485 0.23 +0.3
3,024,096 + BWI76_RS14485 0.34 +0.0
3,024,161 - BWI76_RS14485 0.37 -0.3
3,024,162 + BWI76_RS14485 0.37 +0.2
3,024,188 + BWI76_RS14485 0.39 +0.1
3,024,302 - BWI76_RS14485 0.44 -0.8

Or see this region's nucleotide sequence