Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14475

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14470 and BWI76_RS14475 are separated by 62 nucleotidesBWI76_RS14475 and BWI76_RS14480 are separated by 350 nucleotides BWI76_RS14470: BWI76_RS14470 - L-serine ammonia-lyase, at 3,019,110 to 3,020,477 _RS14470 BWI76_RS14475: BWI76_RS14475 - serine/threonine transporter, at 3,020,540 to 3,021,823 _RS14475 BWI76_RS14480: BWI76_RS14480 - hypothetical protein, at 3,022,174 to 3,023,424 _RS14480 Position (kb) 3020 3021 3022Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 3019.684 kb on + strandat 3019.759 kb on - strand, within BWI76_RS14470at 3019.924 kb on + strand, within BWI76_RS14470at 3019.947 kb on - strand, within BWI76_RS14470at 3019.950 kb on + strand, within BWI76_RS14470at 3020.024 kb on + strand, within BWI76_RS14470at 3020.206 kb on - strand, within BWI76_RS14470at 3020.270 kb on + strand, within BWI76_RS14470at 3020.323 kb on + strand, within BWI76_RS14470at 3020.334 kb on - strandat 3020.361 kb on - strandat 3020.414 kb on + strandat 3020.621 kb on + strandat 3020.834 kb on - strand, within BWI76_RS14475at 3020.904 kb on + strand, within BWI76_RS14475at 3020.946 kb on - strand, within BWI76_RS14475at 3020.975 kb on + strand, within BWI76_RS14475at 3021.074 kb on + strand, within BWI76_RS14475at 3021.074 kb on + strand, within BWI76_RS14475at 3021.082 kb on - strand, within BWI76_RS14475at 3021.249 kb on - strand, within BWI76_RS14475at 3021.257 kb on - strand, within BWI76_RS14475at 3021.257 kb on - strand, within BWI76_RS14475at 3021.259 kb on - strandat 3021.259 kb on - strand, within BWI76_RS14475at 3021.333 kb on + strand, within BWI76_RS14475at 3021.333 kb on + strand, within BWI76_RS14475at 3021.341 kb on - strand, within BWI76_RS14475at 3021.341 kb on - strand, within BWI76_RS14475at 3021.341 kb on - strand, within BWI76_RS14475at 3021.380 kb on - strand, within BWI76_RS14475at 3021.530 kb on + strand, within BWI76_RS14475at 3021.538 kb on - strand, within BWI76_RS14475at 3021.538 kb on - strand, within BWI76_RS14475at 3021.581 kb on - strand, within BWI76_RS14475at 3021.636 kb on - strand, within BWI76_RS14475at 3021.721 kb on - strandat 3021.726 kb on - strandat 3021.735 kb on + strandat 3021.770 kb on - strandat 3021.857 kb on - strandat 3021.930 kb on - strandat 3022.183 kb on + strandat 3022.189 kb on - strandat 3022.201 kb on - strandat 3022.201 kb on - strandat 3022.329 kb on - strand, within BWI76_RS14480at 3022.421 kb on - strand, within BWI76_RS14480at 3022.484 kb on + strand, within BWI76_RS14480at 3022.592 kb on + strand, within BWI76_RS14480at 3022.698 kb on + strand, within BWI76_RS14480

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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3,019,684 + +3.8
3,019,759 - BWI76_RS14470 0.47 -1.8
3,019,924 + BWI76_RS14470 0.60 -0.1
3,019,947 - BWI76_RS14470 0.61 +0.5
3,019,950 + BWI76_RS14470 0.61 -2.8
3,020,024 + BWI76_RS14470 0.67 -1.1
3,020,206 - BWI76_RS14470 0.80 -1.6
3,020,270 + BWI76_RS14470 0.85 -1.2
3,020,323 + BWI76_RS14470 0.89 -1.2
3,020,334 - -2.5
3,020,361 - -1.9
3,020,414 + +0.8
3,020,621 + +0.7
3,020,834 - BWI76_RS14475 0.23 +0.3
3,020,904 + BWI76_RS14475 0.28 +0.1
3,020,946 - BWI76_RS14475 0.32 -2.7
3,020,975 + BWI76_RS14475 0.34 -1.3
3,021,074 + BWI76_RS14475 0.42 -0.8
3,021,074 + BWI76_RS14475 0.42 +1.6
3,021,082 - BWI76_RS14475 0.42 +0.4
3,021,249 - BWI76_RS14475 0.55 -0.1
3,021,257 - BWI76_RS14475 0.56 -0.2
3,021,257 - BWI76_RS14475 0.56 -2.0
3,021,259 - +0.8
3,021,259 - BWI76_RS14475 0.56 +0.6
3,021,333 + BWI76_RS14475 0.62 +0.8
3,021,333 + BWI76_RS14475 0.62 -0.9
3,021,341 - BWI76_RS14475 0.62 -0.1
3,021,341 - BWI76_RS14475 0.62 +0.5
3,021,341 - BWI76_RS14475 0.62 -0.2
3,021,380 - BWI76_RS14475 0.65 +0.8
3,021,530 + BWI76_RS14475 0.77 -1.8
3,021,538 - BWI76_RS14475 0.78 -0.5
3,021,538 - BWI76_RS14475 0.78 -2.4
3,021,581 - BWI76_RS14475 0.81 -0.5
3,021,636 - BWI76_RS14475 0.85 -0.2
3,021,721 - +0.8
3,021,726 - -0.2
3,021,735 + +0.2
3,021,770 - -1.2
3,021,857 - -1.2
3,021,930 - -0.8
3,022,183 + +1.4
3,022,189 - +0.1
3,022,201 - +0.2
3,022,201 - +1.0
3,022,329 - BWI76_RS14480 0.12 -1.5
3,022,421 - BWI76_RS14480 0.20 +0.6
3,022,484 + BWI76_RS14480 0.25 -0.4
3,022,592 + BWI76_RS14480 0.33 -0.2
3,022,698 + BWI76_RS14480 0.42 -0.2

Or see this region's nucleotide sequence