Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14255

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
2,980,778	+		BWI76_RS14250	0.29	+0.8
2,980,851	-		BWI76_RS14250	0.34	-0.5
2,980,870	-		BWI76_RS14250	0.36	-0.7
2,980,872	-		BWI76_RS14250	0.36	+0.0
2,980,875	+		BWI76_RS14250	0.36	+1.0
2,980,918	+		BWI76_RS14250	0.39	+0.4
2,980,918	+		BWI76_RS14250	0.39	-0.2
2,980,926	-		BWI76_RS14250	0.40	+0.4
2,980,935	-		BWI76_RS14250	0.40	-0.5
2,981,037	-		BWI76_RS14250	0.47	+2.4
2,981,158	-		BWI76_RS14250	0.56	+1.0
2,981,255	+		BWI76_RS14250	0.63	-0.5
2,981,255	+		BWI76_RS14250	0.63	-3.5
2,981,263	-				+0.8
2,981,331	-		BWI76_RS14250	0.68	+0.9
2,981,362	-		BWI76_RS14250	0.70	-0.5
2,981,362	-		BWI76_RS14250	0.70	+0.1
2,981,383	+		BWI76_RS14250	0.72	+0.5
2,981,565	-		BWI76_RS14250	0.85	-1.4
2,981,581	-		BWI76_RS14250	0.86	-0.1
2,981,625	+		BWI76_RS14250	0.89	-0.2
2,981,635	+		BWI76_RS14250	0.90	+1.5
2,981,643	-				-0.5
2,981,697	+				-0.4
2,981,726	+				+0.5
2,981,736	+				+1.2
2,981,744	-				+1.1
2,981,824	+				+0.3
2,982,006	+		BWI76_RS14255	0.23	-0.3
2,982,162	+		BWI76_RS14255	0.39	+0.7
2,982,170	-		BWI76_RS14255	0.39	-1.3
2,982,227	-		BWI76_RS14255	0.45	+0.3
2,982,232	-		BWI76_RS14255	0.46	-1.2
2,982,253	+		BWI76_RS14255	0.48	+0.1
2,982,261	-		BWI76_RS14255	0.48	+0.7
2,982,271	+		BWI76_RS14255	0.49	-0.2
2,982,297	+		BWI76_RS14255	0.52	-0.9
2,982,366	-		BWI76_RS14255	0.59	-1.0
2,982,488	+		BWI76_RS14255	0.71	-0.5
2,982,488	+		BWI76_RS14255	0.71	-0.2
2,982,854	-		BWI76_RS14260	0.51	-0.4
2,982,957	+				-2.9
2,982,974	-				-1.1
2,983,006	-				-1.5
2,983,024	+				-1.5
2,983,032	+				+0.3
2,983,039	-				-1.0
2,983,039	-				+0.3
2,983,055	-				-0.3
2,983,171	+				-0.8
2,983,225	+		BWI76_RS14265	0.14	+1.4
2,983,372	-		BWI76_RS14265	0.30	+1.1
2,983,406	-		BWI76_RS14265	0.34	-1.5
2,983,534	+		BWI76_RS14265	0.47	-1.8
2,983,636	+		BWI76_RS14265	0.58	-1.1
2,983,757	+		BWI76_RS14265	0.71	+0.8
2,983,757	+		BWI76_RS14265	0.71	-1.2
2,983,757	+		BWI76_RS14265	0.71	+0.8
2,983,765	-		BWI76_RS14265	0.72	+1.4