Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13410 and BWI76_RS13415 are separated by 1 nucleotides BWI76_RS13415 and BWI76_RS13420 overlap by 11 nucleotides BWI76_RS13420 and BWI76_RS13425 are separated by 22 nucleotides
BWI76_RS13410: BWI76_RS13410 - polyamine ABC transporter ATP-binding protein, at 2,797,268 to 2,798,278
_RS13410
BWI76_RS13415: BWI76_RS13415 - spermidine/putrescine ABC transporter permease, at 2,798,280 to 2,799,209
_RS13415
BWI76_RS13420: BWI76_RS13420 - ABC transporter permease, at 2,799,199 to 2,800,005
_RS13420
BWI76_RS13425: BWI76_RS13425 - 1-pyrroline dehydrogenase, at 2,800,028 to 2,801,455
_RS13425
Position (kb)
2798
2799
2800 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2797.769 kb on - strand, within BWI76_RS13410 at 2797.780 kb on + strand, within BWI76_RS13410 at 2797.839 kb on + strand, within BWI76_RS13410 at 2798.327 kb on + strand at 2798.475 kb on - strand, within BWI76_RS13415 at 2798.596 kb on + strand, within BWI76_RS13415 at 2798.604 kb on - strand, within BWI76_RS13415 at 2798.693 kb on - strand, within BWI76_RS13415 at 2799.088 kb on + strand, within BWI76_RS13415 at 2799.296 kb on - strand, within BWI76_RS13420 at 2799.351 kb on + strand, within BWI76_RS13420 at 2800.018 kb on + strand at 2800.059 kb on - strand at 2800.147 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,797,769 - BWI76_RS13410 0.50 +0.2 2,797,780 + BWI76_RS13410 0.51 +1.0 2,797,839 + BWI76_RS13410 0.56 +0.3 2,798,327 + +0.2 2,798,475 - BWI76_RS13415 0.21 -0.2 2,798,596 + BWI76_RS13415 0.34 +3.8 2,798,604 - BWI76_RS13415 0.35 +1.3 2,798,693 - BWI76_RS13415 0.44 +0.2 2,799,088 + BWI76_RS13415 0.87 +1.8 2,799,296 - BWI76_RS13420 0.12 +0.4 2,799,351 + BWI76_RS13420 0.19 -1.3 2,800,018 + +1.5 2,800,059 - -2.3 2,800,147 + -0.8
Or see this region's nucleotide sequence