Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12935

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS12925 and BWI76_RS12930 overlap by 4 nucleotidesBWI76_RS12930 and BWI76_RS12935 overlap by 11 nucleotidesBWI76_RS12935 and BWI76_RS12940 are separated by 77 nucleotidesBWI76_RS12940 and BWI76_RS12945 are separated by 57 nucleotides BWI76_RS12925: BWI76_RS12925 - efflux transporter periplasmic adaptor subunit, at 2,692,794 to 2,693,717 _RS12925 BWI76_RS12930: BWI76_RS12930 - DUF1656 domain-containing protein, at 2,693,714 to 2,693,929 _RS12930 BWI76_RS12935: BWI76_RS12935 - fusaric acid resistance protein, at 2,693,919 to 2,695,958 _RS12935 BWI76_RS12940: BWI76_RS12940 - AraC family transcriptional regulator, at 2,696,036 to 2,696,857 _RS12940 BWI76_RS12945: BWI76_RS12945 - AraC family transcriptional regulator, at 2,696,915 to 2,697,310 _RS12945 Position (kb) 2693 2694 2695 2696Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2692.996 kb on - strand, within BWI76_RS12925at 2693.465 kb on + strand, within BWI76_RS12925at 2693.734 kb on - strandat 2694.051 kb on + strandat 2694.369 kb on - strand, within BWI76_RS12935at 2694.511 kb on - strand, within BWI76_RS12935at 2694.527 kb on + strand, within BWI76_RS12935at 2694.980 kb on + strand, within BWI76_RS12935at 2695.368 kb on - strand, within BWI76_RS12935at 2695.368 kb on - strand, within BWI76_RS12935at 2695.485 kb on + strand, within BWI76_RS12935at 2695.493 kb on - strand, within BWI76_RS12935at 2695.854 kb on + strandat 2695.885 kb on + strandat 2695.885 kb on + strandat 2696.060 kb on - strandat 2696.181 kb on + strand, within BWI76_RS12940at 2696.414 kb on + strand, within BWI76_RS12940at 2696.777 kb on - strandat 2696.859 kb on - strandat 2696.859 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,692,996 - BWI76_RS12925 0.22 +1.3
2,693,465 + BWI76_RS12925 0.73 +0.3
2,693,734 - -4.2
2,694,051 + +0.5
2,694,369 - BWI76_RS12935 0.22 +0.5
2,694,511 - BWI76_RS12935 0.29 +0.3
2,694,527 + BWI76_RS12935 0.30 -0.4
2,694,980 + BWI76_RS12935 0.52 +0.4
2,695,368 - BWI76_RS12935 0.71 -0.4
2,695,368 - BWI76_RS12935 0.71 -0.4
2,695,485 + BWI76_RS12935 0.77 -1.5
2,695,493 - BWI76_RS12935 0.77 -0.4
2,695,854 + -0.0
2,695,885 + +0.4
2,695,885 + +0.4
2,696,060 - +0.7
2,696,181 + BWI76_RS12940 0.18 +0.9
2,696,414 + BWI76_RS12940 0.46 -0.9
2,696,777 - +1.1
2,696,859 - -1.6
2,696,859 - +0.6

Or see this region's nucleotide sequence