Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12555 and BWI76_RS12560 overlap by 4 nucleotides BWI76_RS12560 and BWI76_RS12565 overlap by 4 nucleotides BWI76_RS12565 and BWI76_RS12570 overlap by 4 nucleotides BWI76_RS12570 and BWI76_RS12575 are separated by 1 nucleotides
BWI76_RS12550: BWI76_RS12550 - hypothetical protein, at 2,621,981 to 2,622,514
_RS12550
BWI76_RS12555: BWI76_RS12555 - hypothetical protein, at 2,622,511 to 2,623,035
_RS12555
BWI76_RS12560: BWI76_RS12560 - hypothetical protein, at 2,623,032 to 2,623,517
_RS12560
BWI76_RS12565: BWI76_RS12565 - SAM-dependent methyltransferase, at 2,623,514 to 2,624,734
_RS12565
BWI76_RS12570: BWI76_RS12570 - DUF1365 domain-containing protein, at 2,624,731 to 2,625,453
_RS12570
BWI76_RS12575: BWI76_RS12575 - FAD-dependent oxidoreductase, at 2,625,455 to 2,626,714
_RS12575
Position (kb)
2623
2624
2625 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2622.699 kb on - strand, within BWI76_RS12555 at 2622.699 kb on - strand, within BWI76_RS12555 at 2622.806 kb on + strand, within BWI76_RS12555 at 2622.814 kb on - strand, within BWI76_RS12555 at 2622.838 kb on - strand, within BWI76_RS12555 at 2623.224 kb on + strand at 2623.615 kb on + strand at 2623.691 kb on + strand, within BWI76_RS12565 at 2623.995 kb on + strand, within BWI76_RS12565 at 2624.000 kb on + strand, within BWI76_RS12565 at 2624.249 kb on - strand, within BWI76_RS12565 at 2624.267 kb on - strand, within BWI76_RS12565 at 2624.495 kb on - strand, within BWI76_RS12565 at 2624.791 kb on - strand at 2624.865 kb on + strand, within BWI76_RS12570 at 2625.129 kb on + strand, within BWI76_RS12570 at 2625.533 kb on - strand at 2625.567 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,622,699 - BWI76_RS12555 0.36 +0.7 2,622,699 - BWI76_RS12555 0.36 +0.3 2,622,806 + BWI76_RS12555 0.56 -1.5 2,622,814 - BWI76_RS12555 0.58 -0.6 2,622,838 - BWI76_RS12555 0.62 +1.9 2,623,224 + -0.1 2,623,615 + -0.3 2,623,691 + BWI76_RS12565 0.14 +1.6 2,623,995 + BWI76_RS12565 0.39 +1.3 2,624,000 + BWI76_RS12565 0.40 +0.8 2,624,249 - BWI76_RS12565 0.60 +1.0 2,624,267 - BWI76_RS12565 0.62 +0.2 2,624,495 - BWI76_RS12565 0.80 +0.2 2,624,791 - +1.0 2,624,865 + BWI76_RS12570 0.19 +0.1 2,625,129 + BWI76_RS12570 0.55 -2.6 2,625,533 - +0.6 2,625,567 - +0.4
Or see this region's nucleotide sequence