Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS12565

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntBWI76_RS12555 and BWI76_RS12560 overlap by 4 nucleotidesBWI76_RS12560 and BWI76_RS12565 overlap by 4 nucleotidesBWI76_RS12565 and BWI76_RS12570 overlap by 4 nucleotidesBWI76_RS12570 and BWI76_RS12575 are separated by 1 nucleotides BWI76_RS12550: BWI76_RS12550 - hypothetical protein, at 2,621,981 to 2,622,514 _RS12550 BWI76_RS12555: BWI76_RS12555 - hypothetical protein, at 2,622,511 to 2,623,035 _RS12555 BWI76_RS12560: BWI76_RS12560 - hypothetical protein, at 2,623,032 to 2,623,517 _RS12560 BWI76_RS12565: BWI76_RS12565 - SAM-dependent methyltransferase, at 2,623,514 to 2,624,734 _RS12565 BWI76_RS12570: BWI76_RS12570 - DUF1365 domain-containing protein, at 2,624,731 to 2,625,453 _RS12570 BWI76_RS12575: BWI76_RS12575 - FAD-dependent oxidoreductase, at 2,625,455 to 2,626,714 _RS12575 Position (kb) 2623 2624 2625Strain fitness (log2 ratio) -2 -1 0 1 2at 2622.699 kb on - strand, within BWI76_RS12555at 2622.699 kb on - strand, within BWI76_RS12555at 2622.806 kb on + strand, within BWI76_RS12555at 2622.814 kb on - strand, within BWI76_RS12555at 2622.838 kb on - strand, within BWI76_RS12555at 2623.224 kb on + strandat 2623.615 kb on + strandat 2623.691 kb on + strand, within BWI76_RS12565at 2623.995 kb on + strand, within BWI76_RS12565at 2624.000 kb on + strand, within BWI76_RS12565at 2624.249 kb on - strand, within BWI76_RS12565at 2624.267 kb on - strand, within BWI76_RS12565at 2624.495 kb on - strand, within BWI76_RS12565at 2624.791 kb on - strandat 2624.865 kb on + strand, within BWI76_RS12570at 2625.129 kb on + strand, within BWI76_RS12570at 2625.533 kb on - strandat 2625.567 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
remove
2,622,699 - BWI76_RS12555 0.36 +0.7
2,622,699 - BWI76_RS12555 0.36 +0.3
2,622,806 + BWI76_RS12555 0.56 -1.5
2,622,814 - BWI76_RS12555 0.58 -0.6
2,622,838 - BWI76_RS12555 0.62 +1.9
2,623,224 + -0.1
2,623,615 + -0.3
2,623,691 + BWI76_RS12565 0.14 +1.6
2,623,995 + BWI76_RS12565 0.39 +1.3
2,624,000 + BWI76_RS12565 0.40 +0.8
2,624,249 - BWI76_RS12565 0.60 +1.0
2,624,267 - BWI76_RS12565 0.62 +0.2
2,624,495 - BWI76_RS12565 0.80 +0.2
2,624,791 - +1.0
2,624,865 + BWI76_RS12570 0.19 +0.1
2,625,129 + BWI76_RS12570 0.55 -2.6
2,625,533 - +0.6
2,625,567 - +0.4

Or see this region's nucleotide sequence