Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11605

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11600 and BWI76_RS11605 are separated by 4 nucleotidesBWI76_RS11605 and BWI76_RS11610 are separated by 70 nucleotides BWI76_RS11600: BWI76_RS11600 - selenide, water dikinase SelD, at 2,440,978 to 2,442,021 _RS11600 BWI76_RS11605: BWI76_RS11605 - DNA topoisomerase III, at 2,442,026 to 2,443,981 _RS11605 BWI76_RS11610: BWI76_RS11610 - glutamate dehydrogenase, at 2,444,052 to 2,445,395 _RS11610 Position (kb) 2442 2443 2444Strain fitness (log2 ratio) -3 -2 -1 0 1at 2441.556 kb on + strand, within BWI76_RS11600at 2441.564 kb on - strand, within BWI76_RS11600at 2442.212 kb on + strandat 2442.253 kb on + strand, within BWI76_RS11605at 2442.418 kb on - strand, within BWI76_RS11605at 2442.624 kb on + strand, within BWI76_RS11605at 2442.759 kb on + strand, within BWI76_RS11605at 2442.783 kb on - strand, within BWI76_RS11605at 2442.990 kb on - strand, within BWI76_RS11605at 2444.153 kb on - strandat 2444.284 kb on + strand, within BWI76_RS11610at 2444.439 kb on - strand, within BWI76_RS11610at 2444.464 kb on - strand, within BWI76_RS11610

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,441,556 + BWI76_RS11600 0.55 -0.2
2,441,564 - BWI76_RS11600 0.56 -3.5
2,442,212 + -0.8
2,442,253 + BWI76_RS11605 0.12 +0.7
2,442,418 - BWI76_RS11605 0.20 +0.9
2,442,624 + BWI76_RS11605 0.31 -0.7
2,442,759 + BWI76_RS11605 0.37 +1.2
2,442,783 - BWI76_RS11605 0.39 +1.3
2,442,990 - BWI76_RS11605 0.49 +0.2
2,444,153 - -0.7
2,444,284 + BWI76_RS11610 0.17 -1.1
2,444,439 - BWI76_RS11610 0.29 +0.9
2,444,464 - BWI76_RS11610 0.31 -2.8

Or see this region's nucleotide sequence