Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10880 and BWI76_RS10885 are separated by 280 nucleotides BWI76_RS10885 and BWI76_RS10890 are separated by 268 nucleotides BWI76_RS10890 and BWI76_RS10895 are separated by 84 nucleotides BWI76_RS10895 and BWI76_RS10900 are separated by 22 nucleotides
BWI76_RS10880: BWI76_RS10880 - LysR family transcriptional regulator, at 2,299,798 to 2,300,703
_RS10880
BWI76_RS10885: BWI76_RS10885 - tRNA-Ser, at 2,300,984 to 2,301,071
_RS10885
BWI76_RS10890: BWI76_RS10890 - glyoxylate/hydroxypyruvate reductase A, at 2,301,340 to 2,302,278
_RS10890
BWI76_RS10895: BWI76_RS10895 - phosphatase, at 2,302,363 to 2,303,100
_RS10895
BWI76_RS10900: BWI76_RS10900 - putative oxidoreductase, at 2,303,123 to 2,303,677
_RS10900
Position (kb)
2301
2302
2303 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2301.248 kb on - strand at 2301.335 kb on - strand at 2301.722 kb on + strand, within BWI76_RS10890 at 2302.022 kb on + strand, within BWI76_RS10890 at 2302.254 kb on + strand at 2302.370 kb on + strand at 2302.378 kb on - strand at 2302.393 kb on - strand at 2302.487 kb on - strand, within BWI76_RS10895 at 2302.686 kb on + strand, within BWI76_RS10895 at 2302.718 kb on + strand, within BWI76_RS10895 at 2302.897 kb on + strand, within BWI76_RS10895 at 2302.924 kb on + strand, within BWI76_RS10895 at 2303.027 kb on - strand at 2303.148 kb on - strand at 2303.172 kb on - strand at 2303.210 kb on - strand, within BWI76_RS10900
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,301,248 - -0.8 2,301,335 - +0.6 2,301,722 + BWI76_RS10890 0.41 +0.6 2,302,022 + BWI76_RS10890 0.73 +1.1 2,302,254 + +0.2 2,302,370 + -1.5 2,302,378 - -1.2 2,302,393 - -0.8 2,302,487 - BWI76_RS10895 0.17 -0.2 2,302,686 + BWI76_RS10895 0.44 +0.0 2,302,718 + BWI76_RS10895 0.48 +1.8 2,302,897 + BWI76_RS10895 0.72 -1.1 2,302,924 + BWI76_RS10895 0.76 +0.7 2,303,027 - -0.2 2,303,148 - +0.8 2,303,172 - +0.2 2,303,210 - BWI76_RS10900 0.16 -2.0
Or see this region's nucleotide sequence