Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10820

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10815 and BWI76_RS10820 are separated by 52 nucleotidesBWI76_RS10820 and BWI76_RS10825 are separated by 4 nucleotides BWI76_RS10815: BWI76_RS10815 - iron permease, at 2,285,413 to 2,286,243 _RS10815 BWI76_RS10820: BWI76_RS10820 - iron uptake system protein EfeO, at 2,286,296 to 2,287,423 _RS10820 BWI76_RS10825: BWI76_RS10825 - deferrochelatase/peroxidase EfeB, at 2,287,428 to 2,288,708 _RS10825 Position (kb) 2286 2287 2288Strain fitness (log2 ratio) -1 0 1 2at 2285.566 kb on - strand, within BWI76_RS10815at 2285.663 kb on + strand, within BWI76_RS10815at 2285.812 kb on - strand, within BWI76_RS10815at 2286.017 kb on + strand, within BWI76_RS10815at 2286.228 kb on + strandat 2286.346 kb on + strandat 2286.677 kb on + strand, within BWI76_RS10820at 2286.695 kb on - strand, within BWI76_RS10820at 2286.761 kb on + strand, within BWI76_RS10820at 2286.807 kb on - strand, within BWI76_RS10820at 2287.341 kb on - strandat 2287.424 kb on + strandat 2287.655 kb on - strand, within BWI76_RS10825at 2287.747 kb on - strand, within BWI76_RS10825at 2288.336 kb on - strand, within BWI76_RS10825

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,285,566 - BWI76_RS10815 0.18 +1.5
2,285,663 + BWI76_RS10815 0.30 +1.0
2,285,812 - BWI76_RS10815 0.48 -0.5
2,286,017 + BWI76_RS10815 0.73 +1.3
2,286,228 + -0.8
2,286,346 + +2.1
2,286,677 + BWI76_RS10820 0.34 +1.6
2,286,695 - BWI76_RS10820 0.35 +1.9
2,286,761 + BWI76_RS10820 0.41 +0.6
2,286,807 - BWI76_RS10820 0.45 -0.8
2,287,341 - -0.2
2,287,424 + +1.0
2,287,655 - BWI76_RS10825 0.18 +0.9
2,287,747 - BWI76_RS10825 0.25 -0.0
2,288,336 - BWI76_RS10825 0.71 +1.1

Or see this region's nucleotide sequence