Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10400 and BWI76_RS10405 are separated by 125 nucleotides BWI76_RS10405 and BWI76_RS10410 are separated by 234 nucleotides BWI76_RS10410 and BWI76_RS10415 are separated by 91 nucleotides BWI76_RS10415 and BWI76_RS10420 are separated by 190 nucleotides
BWI76_RS10400: BWI76_RS10400 - hypothetical protein, at 2,201,675 to 2,201,860
_RS10400
BWI76_RS10405: BWI76_RS10405 - cold-shock protein, at 2,201,986 to 2,202,207
_RS10405
BWI76_RS10410: BWI76_RS10410 - hypothetical protein, at 2,202,442 to 2,202,702
_RS10410
BWI76_RS10415: BWI76_RS10415 - endoribonuclease L-PSP, at 2,202,794 to 2,203,171
_RS10415
BWI76_RS10420: BWI76_RS10420 - monooxygenase, at 2,203,362 to 2,204,390
_RS10420
Position (kb)
2202
2203
2204 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2201.877 kb on + strand at 2201.933 kb on + strand at 2201.936 kb on - strand at 2201.941 kb on - strand at 2201.941 kb on - strand at 2201.941 kb on - strand at 2201.941 kb on - strand at 2201.965 kb on - strand at 2201.972 kb on + strand at 2201.986 kb on - strand at 2202.004 kb on + strand at 2202.007 kb on + strand at 2202.012 kb on - strand, within BWI76_RS10405 at 2202.017 kb on + strand, within BWI76_RS10405 at 2202.025 kb on - strand, within BWI76_RS10405 at 2202.027 kb on + strand, within BWI76_RS10405 at 2202.071 kb on - strand, within BWI76_RS10405 at 2202.082 kb on - strand, within BWI76_RS10405 at 2202.084 kb on + strand, within BWI76_RS10405 at 2202.086 kb on + strand, within BWI76_RS10405 at 2202.150 kb on + strand, within BWI76_RS10405 at 2202.153 kb on + strand, within BWI76_RS10405 at 2202.160 kb on + strand, within BWI76_RS10405 at 2202.161 kb on - strand, within BWI76_RS10405 at 2202.161 kb on - strand, within BWI76_RS10405 at 2202.170 kb on + strand, within BWI76_RS10405 at 2202.171 kb on - strand, within BWI76_RS10405 at 2202.175 kb on + strand, within BWI76_RS10405 at 2202.183 kb on - strand, within BWI76_RS10405 at 2202.183 kb on - strand, within BWI76_RS10405 at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.241 kb on - strand at 2202.241 kb on - strand at 2202.254 kb on + strand at 2202.268 kb on + strand at 2202.273 kb on + strand at 2202.284 kb on + strand at 2202.299 kb on - strand at 2202.309 kb on + strand at 2202.309 kb on + strand at 2202.389 kb on + strand at 2202.394 kb on + strand at 2202.394 kb on + strand at 2202.442 kb on - strand at 2202.498 kb on - strand, within BWI76_RS10410 at 2202.610 kb on - strand, within BWI76_RS10410 at 2202.806 kb on - strand at 2202.835 kb on + strand, within BWI76_RS10415 at 2202.845 kb on + strand, within BWI76_RS10415 at 2202.979 kb on + strand, within BWI76_RS10415 at 2202.979 kb on + strand, within BWI76_RS10415 at 2203.222 kb on - strand at 2203.281 kb on + strand at 2203.281 kb on + strand at 2203.289 kb on - strand at 2203.289 kb on - strand at 2203.625 kb on + strand, within BWI76_RS10420 at 2203.633 kb on - strand, within BWI76_RS10420 at 2203.633 kb on - strand, within BWI76_RS10420 at 2203.672 kb on + strand, within BWI76_RS10420 at 2203.729 kb on + strand, within BWI76_RS10420 at 2203.880 kb on + strand, within BWI76_RS10420
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,201,877 + -0.8 2,201,933 + +0.6 2,201,936 - +0.8 2,201,941 - +0.5 2,201,941 - -3.9 2,201,941 - -3.4 2,201,941 - -1.6 2,201,965 - -0.8 2,201,972 + -1.8 2,201,986 - -1.6 2,202,004 + +0.6 2,202,007 + -1.6 2,202,012 - BWI76_RS10405 0.12 -1.2 2,202,017 + BWI76_RS10405 0.14 -0.8 2,202,025 - BWI76_RS10405 0.18 -1.6 2,202,027 + BWI76_RS10405 0.18 -1.2 2,202,071 - BWI76_RS10405 0.38 -0.0 2,202,082 - BWI76_RS10405 0.43 +0.6 2,202,084 + BWI76_RS10405 0.44 -0.2 2,202,086 + BWI76_RS10405 0.45 -1.1 2,202,150 + BWI76_RS10405 0.74 -0.7 2,202,153 + BWI76_RS10405 0.75 -4.2 2,202,160 + BWI76_RS10405 0.78 -1.2 2,202,161 - BWI76_RS10405 0.79 -0.8 2,202,161 - BWI76_RS10405 0.79 +1.3 2,202,170 + BWI76_RS10405 0.83 -0.6 2,202,171 - BWI76_RS10405 0.83 -1.6 2,202,175 + BWI76_RS10405 0.85 +1.0 2,202,183 - BWI76_RS10405 0.89 -0.1 2,202,183 - BWI76_RS10405 0.89 -0.6 2,202,185 + -1.1 2,202,185 + +2.3 2,202,185 + +0.3 2,202,185 + +0.8 2,202,185 + -0.1 2,202,185 + +0.5 2,202,185 + -1.1 2,202,193 - -0.3 2,202,193 - -2.1 2,202,193 - +0.7 2,202,193 - +0.2 2,202,193 - -0.4 2,202,193 - -1.1 2,202,193 - +1.6 2,202,193 - -2.8 2,202,193 - -0.6 2,202,193 - -1.4 2,202,193 - +0.3 2,202,193 - -1.2 2,202,193 - +0.5 2,202,233 + +0.5 2,202,233 + -0.7 2,202,233 + +0.8 2,202,233 + -0.0 2,202,233 + +0.6 2,202,241 - -2.8 2,202,241 - -1.2 2,202,254 + +0.6 2,202,268 + -0.1 2,202,273 + +1.0 2,202,284 + -0.8 2,202,299 - +0.8 2,202,309 + -2.8 2,202,309 + +0.2 2,202,389 + -3.7 2,202,394 + -1.0 2,202,394 + +0.9 2,202,442 - +0.5 2,202,498 - BWI76_RS10410 0.21 +0.8 2,202,610 - BWI76_RS10410 0.64 -1.2 2,202,806 - +0.4 2,202,835 + BWI76_RS10415 0.11 -1.1 2,202,845 + BWI76_RS10415 0.13 -0.6 2,202,979 + BWI76_RS10415 0.49 -0.6 2,202,979 + BWI76_RS10415 0.49 -1.5 2,203,222 - +0.8 2,203,281 + +0.8 2,203,281 + -1.6 2,203,289 - +0.9 2,203,289 - +0.9 2,203,625 + BWI76_RS10420 0.26 +0.4 2,203,633 - BWI76_RS10420 0.26 -1.8 2,203,633 - BWI76_RS10420 0.26 +0.8 2,203,672 + BWI76_RS10420 0.30 +1.2 2,203,729 + BWI76_RS10420 0.36 +1.2 2,203,880 + BWI76_RS10420 0.50 +0.1
Or see this region's nucleotide sequence