Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS10415

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS10400 and BWI76_RS10405 are separated by 125 nucleotidesBWI76_RS10405 and BWI76_RS10410 are separated by 234 nucleotidesBWI76_RS10410 and BWI76_RS10415 are separated by 91 nucleotidesBWI76_RS10415 and BWI76_RS10420 are separated by 190 nucleotides BWI76_RS10400: BWI76_RS10400 - hypothetical protein, at 2,201,675 to 2,201,860 _RS10400 BWI76_RS10405: BWI76_RS10405 - cold-shock protein, at 2,201,986 to 2,202,207 _RS10405 BWI76_RS10410: BWI76_RS10410 - hypothetical protein, at 2,202,442 to 2,202,702 _RS10410 BWI76_RS10415: BWI76_RS10415 - endoribonuclease L-PSP, at 2,202,794 to 2,203,171 _RS10415 BWI76_RS10420: BWI76_RS10420 - monooxygenase, at 2,203,362 to 2,204,390 _RS10420 Position (kb) 2202 2203 2204Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2201.877 kb on + strandat 2201.933 kb on + strandat 2201.936 kb on - strandat 2201.941 kb on - strandat 2201.941 kb on - strandat 2201.941 kb on - strandat 2201.941 kb on - strandat 2201.965 kb on - strandat 2201.972 kb on + strandat 2201.986 kb on - strandat 2202.004 kb on + strandat 2202.007 kb on + strandat 2202.012 kb on - strand, within BWI76_RS10405at 2202.017 kb on + strand, within BWI76_RS10405at 2202.025 kb on - strand, within BWI76_RS10405at 2202.027 kb on + strand, within BWI76_RS10405at 2202.071 kb on - strand, within BWI76_RS10405at 2202.082 kb on - strand, within BWI76_RS10405at 2202.084 kb on + strand, within BWI76_RS10405at 2202.086 kb on + strand, within BWI76_RS10405at 2202.150 kb on + strand, within BWI76_RS10405at 2202.153 kb on + strand, within BWI76_RS10405at 2202.160 kb on + strand, within BWI76_RS10405at 2202.161 kb on - strand, within BWI76_RS10405at 2202.161 kb on - strand, within BWI76_RS10405at 2202.170 kb on + strand, within BWI76_RS10405at 2202.171 kb on - strand, within BWI76_RS10405at 2202.175 kb on + strand, within BWI76_RS10405at 2202.183 kb on - strand, within BWI76_RS10405at 2202.183 kb on - strand, within BWI76_RS10405at 2202.185 kb on + strandat 2202.185 kb on + strandat 2202.185 kb on + strandat 2202.185 kb on + strandat 2202.185 kb on + strandat 2202.185 kb on + strandat 2202.185 kb on + strandat 2202.193 kb on - strandat 2202.193 kb on - strandat 2202.193 kb on - strandat 2202.193 kb on - strandat 2202.193 kb on - strandat 2202.193 kb on - strandat 2202.193 kb on - strandat 2202.193 kb on - strandat 2202.193 kb on - strandat 2202.193 kb on - strandat 2202.193 kb on - strandat 2202.193 kb on - strandat 2202.193 kb on - strandat 2202.233 kb on + strandat 2202.233 kb on + strandat 2202.233 kb on + strandat 2202.233 kb on + strandat 2202.233 kb on + strandat 2202.241 kb on - strandat 2202.241 kb on - strandat 2202.254 kb on + strandat 2202.268 kb on + strandat 2202.273 kb on + strandat 2202.284 kb on + strandat 2202.299 kb on - strandat 2202.309 kb on + strandat 2202.309 kb on + strandat 2202.389 kb on + strandat 2202.394 kb on + strandat 2202.394 kb on + strandat 2202.442 kb on - strandat 2202.498 kb on - strand, within BWI76_RS10410at 2202.610 kb on - strand, within BWI76_RS10410at 2202.806 kb on - strandat 2202.835 kb on + strand, within BWI76_RS10415at 2202.845 kb on + strand, within BWI76_RS10415at 2202.979 kb on + strand, within BWI76_RS10415at 2202.979 kb on + strand, within BWI76_RS10415at 2203.222 kb on - strandat 2203.281 kb on + strandat 2203.281 kb on + strandat 2203.289 kb on - strandat 2203.289 kb on - strandat 2203.625 kb on + strand, within BWI76_RS10420at 2203.633 kb on - strand, within BWI76_RS10420at 2203.633 kb on - strand, within BWI76_RS10420at 2203.672 kb on + strand, within BWI76_RS10420at 2203.729 kb on + strand, within BWI76_RS10420at 2203.880 kb on + strand, within BWI76_RS10420

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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2,201,877 + -0.8
2,201,933 + +0.6
2,201,936 - +0.8
2,201,941 - +0.5
2,201,941 - -3.9
2,201,941 - -3.4
2,201,941 - -1.6
2,201,965 - -0.8
2,201,972 + -1.8
2,201,986 - -1.6
2,202,004 + +0.6
2,202,007 + -1.6
2,202,012 - BWI76_RS10405 0.12 -1.2
2,202,017 + BWI76_RS10405 0.14 -0.8
2,202,025 - BWI76_RS10405 0.18 -1.6
2,202,027 + BWI76_RS10405 0.18 -1.2
2,202,071 - BWI76_RS10405 0.38 -0.0
2,202,082 - BWI76_RS10405 0.43 +0.6
2,202,084 + BWI76_RS10405 0.44 -0.2
2,202,086 + BWI76_RS10405 0.45 -1.1
2,202,150 + BWI76_RS10405 0.74 -0.7
2,202,153 + BWI76_RS10405 0.75 -4.2
2,202,160 + BWI76_RS10405 0.78 -1.2
2,202,161 - BWI76_RS10405 0.79 -0.8
2,202,161 - BWI76_RS10405 0.79 +1.3
2,202,170 + BWI76_RS10405 0.83 -0.6
2,202,171 - BWI76_RS10405 0.83 -1.6
2,202,175 + BWI76_RS10405 0.85 +1.0
2,202,183 - BWI76_RS10405 0.89 -0.1
2,202,183 - BWI76_RS10405 0.89 -0.6
2,202,185 + -1.1
2,202,185 + +2.3
2,202,185 + +0.3
2,202,185 + +0.8
2,202,185 + -0.1
2,202,185 + +0.5
2,202,185 + -1.1
2,202,193 - -0.3
2,202,193 - -2.1
2,202,193 - +0.7
2,202,193 - +0.2
2,202,193 - -0.4
2,202,193 - -1.1
2,202,193 - +1.6
2,202,193 - -2.8
2,202,193 - -0.6
2,202,193 - -1.4
2,202,193 - +0.3
2,202,193 - -1.2
2,202,193 - +0.5
2,202,233 + +0.5
2,202,233 + -0.7
2,202,233 + +0.8
2,202,233 + -0.0
2,202,233 + +0.6
2,202,241 - -2.8
2,202,241 - -1.2
2,202,254 + +0.6
2,202,268 + -0.1
2,202,273 + +1.0
2,202,284 + -0.8
2,202,299 - +0.8
2,202,309 + -2.8
2,202,309 + +0.2
2,202,389 + -3.7
2,202,394 + -1.0
2,202,394 + +0.9
2,202,442 - +0.5
2,202,498 - BWI76_RS10410 0.21 +0.8
2,202,610 - BWI76_RS10410 0.64 -1.2
2,202,806 - +0.4
2,202,835 + BWI76_RS10415 0.11 -1.1
2,202,845 + BWI76_RS10415 0.13 -0.6
2,202,979 + BWI76_RS10415 0.49 -0.6
2,202,979 + BWI76_RS10415 0.49 -1.5
2,203,222 - +0.8
2,203,281 + +0.8
2,203,281 + -1.6
2,203,289 - +0.9
2,203,289 - +0.9
2,203,625 + BWI76_RS10420 0.26 +0.4
2,203,633 - BWI76_RS10420 0.26 -1.8
2,203,633 - BWI76_RS10420 0.26 +0.8
2,203,672 + BWI76_RS10420 0.30 +1.2
2,203,729 + BWI76_RS10420 0.36 +1.2
2,203,880 + BWI76_RS10420 0.50 +0.1

Or see this region's nucleotide sequence