Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10265 and BWI76_RS10270 overlap by 4 nucleotides BWI76_RS10270 and BWI76_RS10275 are separated by 13 nucleotides BWI76_RS10275 and BWI76_RS10280 overlap by 4 nucleotides
BWI76_RS10265: BWI76_RS10265 - mandelate racemase/muconate lactonizing protein, at 2,174,048 to 2,175,148
_RS10265
BWI76_RS10270: BWI76_RS10270 - acetylornithine deacetylase, at 2,175,145 to 2,176,320
_RS10270
BWI76_RS10275: BWI76_RS10275 - ABC transporter, at 2,176,334 to 2,177,074
_RS10275
BWI76_RS10280: BWI76_RS10280 - polar amino acid ABC transporter permease, at 2,177,071 to 2,177,796
_RS10280
Position (kb)
2175
2176
2177 Strain fitness (log2 ratio)
-2
-1
0
1 at 2174.399 kb on + strand, within BWI76_RS10265 at 2174.485 kb on - strand, within BWI76_RS10265 at 2174.485 kb on - strand, within BWI76_RS10265 at 2174.799 kb on - strand, within BWI76_RS10265 at 2174.854 kb on - strand, within BWI76_RS10265 at 2174.904 kb on - strand, within BWI76_RS10265 at 2175.283 kb on - strand, within BWI76_RS10270 at 2175.320 kb on - strand, within BWI76_RS10270 at 2175.825 kb on - strand, within BWI76_RS10270 at 2176.089 kb on - strand, within BWI76_RS10270 at 2176.116 kb on - strand, within BWI76_RS10270 at 2176.353 kb on + strand at 2176.494 kb on - strand, within BWI76_RS10275 at 2176.517 kb on - strand, within BWI76_RS10275 at 2176.813 kb on + strand, within BWI76_RS10275 at 2176.990 kb on + strand, within BWI76_RS10275 at 2177.183 kb on + strand, within BWI76_RS10280 at 2177.191 kb on - strand, within BWI76_RS10280
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 2,174,399 + BWI76_RS10265 0.32 +1.0 2,174,485 - BWI76_RS10265 0.40 +0.5 2,174,485 - BWI76_RS10265 0.40 +0.9 2,174,799 - BWI76_RS10265 0.68 -0.4 2,174,854 - BWI76_RS10265 0.73 -0.2 2,174,904 - BWI76_RS10265 0.78 +0.4 2,175,283 - BWI76_RS10270 0.12 +1.6 2,175,320 - BWI76_RS10270 0.15 +1.2 2,175,825 - BWI76_RS10270 0.58 -0.1 2,176,089 - BWI76_RS10270 0.80 +0.9 2,176,116 - BWI76_RS10270 0.83 +0.5 2,176,353 + -2.3 2,176,494 - BWI76_RS10275 0.22 -0.1 2,176,517 - BWI76_RS10275 0.25 +0.4 2,176,813 + BWI76_RS10275 0.65 -0.2 2,176,990 + BWI76_RS10275 0.89 +0.2 2,177,183 + BWI76_RS10280 0.15 +1.3 2,177,191 - BWI76_RS10280 0.17 -2.2
Or see this region's nucleotide sequence