Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09465 and BWI76_RS09470 are separated by 10 nucleotides BWI76_RS09470 and BWI76_RS09475 overlap by 4 nucleotides BWI76_RS09475 and BWI76_RS09480 are separated by 57 nucleotides BWI76_RS09480 and BWI76_RS09485 are separated by 42 nucleotides
BWI76_RS09465: BWI76_RS09465 - polyamine ABC transporter ATP-binding protein, at 1,981,746 to 1,982,924
_RS09465
BWI76_RS09470: BWI76_RS09470 - putrescine ABC transporter permease, at 1,982,935 to 1,983,888
_RS09470
BWI76_RS09475: BWI76_RS09475 - putrescine ABC transporter permease PotI, at 1,983,885 to 1,984,730
_RS09475
BWI76_RS09480: BWI76_RS09480 - hypothetical protein, at 1,984,788 to 1,985,276
_RS09480
BWI76_RS09485: BWI76_RS09485 - 23S rRNA (uracil(747)-C(5))-methyltransferase, at 1,985,319 to 1,986,449
_RS09485
Position (kb) 1983
1984
1985 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1983.548 kb on - strand, within BWI76_RS09470 at 1983.640 kb on - strand, within BWI76_RS09470 at 1983.739 kb on + strand, within BWI76_RS09470 at 1983.802 kb on - strand at 1984.331 kb on + strand, within BWI76_RS09475 at 1984.339 kb on - strand, within BWI76_RS09475 at 1984.378 kb on + strand, within BWI76_RS09475 at 1984.495 kb on + strand, within BWI76_RS09475 at 1984.503 kb on - strand, within BWI76_RS09475 at 1984.710 kb on - strand at 1984.777 kb on - strand at 1984.847 kb on - strand, within BWI76_RS09480 at 1984.859 kb on + strand, within BWI76_RS09480 at 1984.973 kb on - strand, within BWI76_RS09480 at 1984.973 kb on - strand, within BWI76_RS09480 at 1985.132 kb on + strand, within BWI76_RS09480 at 1985.196 kb on - strand, within BWI76_RS09480 at 1985.279 kb on + strand at 1985.455 kb on - strand, within BWI76_RS09485 at 1985.681 kb on + strand, within BWI76_RS09485 at 1985.690 kb on - strand, within BWI76_RS09485
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,983,548 - BWI76_RS09470 0.64 -0.8 1,983,640 - BWI76_RS09470 0.74 +0.9 1,983,739 + BWI76_RS09470 0.84 +0.5 1,983,802 - +2.5 1,984,331 + BWI76_RS09475 0.53 +1.9 1,984,339 - BWI76_RS09475 0.54 -0.2 1,984,378 + BWI76_RS09475 0.58 +1.6 1,984,495 + BWI76_RS09475 0.72 +0.4 1,984,503 - BWI76_RS09475 0.73 +0.6 1,984,710 - +0.7 1,984,777 - +0.1 1,984,847 - BWI76_RS09480 0.12 +0.8 1,984,859 + BWI76_RS09480 0.15 +0.8 1,984,973 - BWI76_RS09480 0.38 +0.1 1,984,973 - BWI76_RS09480 0.38 -0.8 1,985,132 + BWI76_RS09480 0.70 +1.2 1,985,196 - BWI76_RS09480 0.83 -2.0 1,985,279 + +0.2 1,985,455 - BWI76_RS09485 0.12 +0.6 1,985,681 + BWI76_RS09485 0.32 +0.7 1,985,690 - BWI76_RS09485 0.33 +1.6
Or see this region's nucleotide sequence