Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08735 and BWI76_RS08740 overlap by 4 nucleotides BWI76_RS08740 and BWI76_RS08745 are separated by 100 nucleotides
BWI76_RS08735: BWI76_RS08735 - Urocanate hydratase (EC 4.2.1.49) (from data) , at 1,839,699 to 1,841,387
_RS08735
BWI76_RS08740: BWI76_RS08740 - Histidine ammonia-lyase (EC 4.3.1.3) (from data) , at 1,841,384 to 1,842,907
_RS08740
BWI76_RS08745: BWI76_RS08745 - proline-specific permease, at 1,843,008 to 1,844,387
_RS08745
Position (kb)
1841
1842
1843 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1840.386 kb on + strand, within BWI76_RS08735 at 1840.558 kb on - strand, within BWI76_RS08735 at 1840.895 kb on + strand, within BWI76_RS08735 at 1841.111 kb on + strand, within BWI76_RS08735 at 1841.181 kb on - strand, within BWI76_RS08735 at 1841.844 kb on + strand, within BWI76_RS08740 at 1841.968 kb on + strand, within BWI76_RS08740 at 1842.064 kb on - strand, within BWI76_RS08740 at 1842.136 kb on + strand at 1842.263 kb on + strand, within BWI76_RS08740 at 1842.272 kb on - strand, within BWI76_RS08740 at 1842.482 kb on - strand, within BWI76_RS08740 at 1842.597 kb on + strand, within BWI76_RS08740 at 1843.359 kb on - strand, within BWI76_RS08745 at 1843.361 kb on + strand, within BWI76_RS08745 at 1843.748 kb on + strand, within BWI76_RS08745
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 1,840,386 + BWI76_RS08735 0.41 +0.5 1,840,558 - BWI76_RS08735 0.51 +1.4 1,840,895 + BWI76_RS08735 0.71 +0.2 1,841,111 + BWI76_RS08735 0.84 -0.6 1,841,181 - BWI76_RS08735 0.88 -0.1 1,841,844 + BWI76_RS08740 0.30 -1.2 1,841,968 + BWI76_RS08740 0.38 -0.0 1,842,064 - BWI76_RS08740 0.45 -1.1 1,842,136 + +0.8 1,842,263 + BWI76_RS08740 0.58 +0.8 1,842,272 - BWI76_RS08740 0.58 -2.8 1,842,482 - BWI76_RS08740 0.72 +0.7 1,842,597 + BWI76_RS08740 0.80 -0.5 1,843,359 - BWI76_RS08745 0.25 -0.5 1,843,361 + BWI76_RS08745 0.26 +0.6 1,843,748 + BWI76_RS08745 0.54 -2.2
Or see this region's nucleotide sequence