Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08380

Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08370 and BWI76_RS08375 are separated by 9 nucleotidesBWI76_RS08375 and BWI76_RS08380 are separated by 15 nucleotidesBWI76_RS08380 and BWI76_RS08385 are separated by 45 nucleotides BWI76_RS08370: BWI76_RS08370 - membrane protein, at 1,771,176 to 1,772,171 _RS08370 BWI76_RS08375: BWI76_RS08375 - pyroglutamyl-peptidase I, at 1,772,181 to 1,772,825 _RS08375 BWI76_RS08380: BWI76_RS08380 - endonuclease VIII, at 1,772,841 to 1,773,632 _RS08380 BWI76_RS08385: BWI76_RS08385 - citrate (Si)-synthase, at 1,773,678 to 1,774,961 _RS08385 Position (kb) 1772 1773 1774Strain fitness (log2 ratio) -2 -1 0 1at 1772.016 kb on + strand, within BWI76_RS08370at 1772.108 kb on + strandat 1772.116 kb on + strandat 1772.627 kb on - strand, within BWI76_RS08375at 1772.633 kb on + strand, within BWI76_RS08375at 1772.904 kb on + strandat 1772.912 kb on - strandat 1773.069 kb on + strand, within BWI76_RS08380at 1773.220 kb on + strand, within BWI76_RS08380at 1773.794 kb on + strandat 1773.862 kb on - strand, within BWI76_RS08385at 1774.346 kb on + strand, within BWI76_RS08385at 1774.604 kb on - strand, within BWI76_RS08385

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
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1,772,016 + BWI76_RS08370 0.84 +0.3
1,772,108 + +0.2
1,772,116 + +0.8
1,772,627 - BWI76_RS08375 0.69 +0.3
1,772,633 + BWI76_RS08375 0.70 +1.0
1,772,904 + +0.0
1,772,912 - +0.5
1,773,069 + BWI76_RS08380 0.29 +1.1
1,773,220 + BWI76_RS08380 0.48 +1.3
1,773,794 + +0.7
1,773,862 - BWI76_RS08385 0.14 -2.3
1,774,346 + BWI76_RS08385 0.52 -1.9
1,774,604 - BWI76_RS08385 0.72 -1.9

Or see this region's nucleotide sequence