Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04615 and BWI76_RS04620 are separated by 162 nucleotides BWI76_RS04620 and BWI76_RS04625 are separated by 87 nucleotides BWI76_RS04625 and BWI76_RS04630 are separated by 231 nucleotides
BWI76_RS04615: BWI76_RS04615 - thiamine transporter substrate binding subunit, at 960,777 to 961,760
_RS04615
BWI76_RS04620: BWI76_RS04620 - transcriptional regulator SgrR, at 961,923 to 963,578
_RS04620
BWI76_RS04625: BWI76_RS04625 - glucose uptake inhibitor SgrT, at 963,666 to 963,818
_RS04625
BWI76_RS04630: BWI76_RS04630 - sugar efflux transporter SetB, at 964,050 to 965,234
_RS04630
Position (kb) 961
962
963
964 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 961.024 kb on + strand, within BWI76_RS04615 at 961.132 kb on - strand, within BWI76_RS04615 at 961.132 kb on - strand, within BWI76_RS04615 at 961.321 kb on + strand, within BWI76_RS04615 at 961.329 kb on - strand, within BWI76_RS04615 at 961.495 kb on + strand, within BWI76_RS04615 at 961.674 kb on - strand at 962.083 kb on + strand at 962.166 kb on - strand, within BWI76_RS04620 at 962.225 kb on - strand, within BWI76_RS04620 at 962.234 kb on + strand, within BWI76_RS04620 at 962.615 kb on + strand, within BWI76_RS04620 at 962.759 kb on + strand, within BWI76_RS04620 at 962.761 kb on + strand, within BWI76_RS04620 at 962.837 kb on - strand, within BWI76_RS04620 at 962.885 kb on + strand, within BWI76_RS04620 at 962.885 kb on + strand, within BWI76_RS04620 at 963.194 kb on + strand, within BWI76_RS04620 at 963.230 kb on + strand, within BWI76_RS04620 at 963.364 kb on + strand, within BWI76_RS04620 at 963.364 kb on + strand, within BWI76_RS04620 at 963.397 kb on + strand, within BWI76_RS04620 at 963.774 kb on + strand, within BWI76_RS04625 at 963.785 kb on + strand, within BWI76_RS04625 at 963.805 kb on + strand at 963.805 kb on + strand at 963.813 kb on - strand at 963.827 kb on + strand at 963.827 kb on + strand at 963.827 kb on + strand at 963.975 kb on + strand at 963.975 kb on + strand at 963.975 kb on + strand at 964.159 kb on + strand at 964.203 kb on + strand, within BWI76_RS04630 at 964.208 kb on + strand, within BWI76_RS04630 at 964.246 kb on + strand, within BWI76_RS04630 at 964.263 kb on + strand, within BWI76_RS04630 at 964.365 kb on - strand, within BWI76_RS04630 at 964.365 kb on - strand, within BWI76_RS04630 at 964.386 kb on - strand, within BWI76_RS04630 at 964.386 kb on - strand, within BWI76_RS04630 at 964.464 kb on + strand, within BWI76_RS04630 at 964.496 kb on + strand, within BWI76_RS04630 at 964.524 kb on + strand, within BWI76_RS04630
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 961,024 + BWI76_RS04615 0.25 +0.2 961,132 - BWI76_RS04615 0.36 -0.0 961,132 - BWI76_RS04615 0.36 +0.2 961,321 + BWI76_RS04615 0.55 -0.2 961,329 - BWI76_RS04615 0.56 -2.7 961,495 + BWI76_RS04615 0.73 +1.0 961,674 - +0.4 962,083 + +1.2 962,166 - BWI76_RS04620 0.15 +1.4 962,225 - BWI76_RS04620 0.18 +0.5 962,234 + BWI76_RS04620 0.19 -1.7 962,615 + BWI76_RS04620 0.42 +1.2 962,759 + BWI76_RS04620 0.50 +0.5 962,761 + BWI76_RS04620 0.51 +1.7 962,837 - BWI76_RS04620 0.55 -0.4 962,885 + BWI76_RS04620 0.58 +1.1 962,885 + BWI76_RS04620 0.58 +3.1 963,194 + BWI76_RS04620 0.77 -2.4 963,230 + BWI76_RS04620 0.79 +2.9 963,364 + BWI76_RS04620 0.87 +0.8 963,364 + BWI76_RS04620 0.87 +1.0 963,397 + BWI76_RS04620 0.89 +0.9 963,774 + BWI76_RS04625 0.71 +1.0 963,785 + BWI76_RS04625 0.78 -0.0 963,805 + +0.6 963,805 + -0.1 963,813 - -1.7 963,827 + -0.1 963,827 + -3.8 963,827 + +0.3 963,975 + +0.4 963,975 + +0.1 963,975 + +0.2 964,159 + +1.4 964,203 + BWI76_RS04630 0.13 -0.3 964,208 + BWI76_RS04630 0.13 +0.6 964,246 + BWI76_RS04630 0.17 -0.7 964,263 + BWI76_RS04630 0.18 +0.8 964,365 - BWI76_RS04630 0.27 -0.1 964,365 - BWI76_RS04630 0.27 +0.2 964,386 - BWI76_RS04630 0.28 +0.1 964,386 - BWI76_RS04630 0.28 -3.3 964,464 + BWI76_RS04630 0.35 +1.1 964,496 + BWI76_RS04630 0.38 +3.0 964,524 + BWI76_RS04630 0.40 -0.5
Or see this region's nucleotide sequence