Experiment: RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00855 and BWI76_RS00860 are separated by 31 nucleotides BWI76_RS00860 and BWI76_RS00865 overlap by 1 nucleotides BWI76_RS00865 and BWI76_RS00870 overlap by 18 nucleotides
BWI76_RS00855: BWI76_RS00855 - argininosuccinate lyase, at 176,786 to 178,159
_RS00855
BWI76_RS00860: BWI76_RS00860 - hypothetical protein, at 178,191 to 178,430
_RS00860
BWI76_RS00865: BWI76_RS00865 - DNA-binding transcriptional regulator OxyR, at 178,430 to 179,347
_RS00865
BWI76_RS00870: BWI76_RS00870 - NAD(P)(+) transhydrogenase, at 179,330 to 180,730
_RS00870
Position (kb) 178
179
180 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 177.458 kb on + strand, within BWI76_RS00855 at 177.466 kb on - strand, within BWI76_RS00855 at 177.598 kb on + strand, within BWI76_RS00855 at 177.712 kb on + strand, within BWI76_RS00855 at 177.719 kb on - strand, within BWI76_RS00855 at 177.744 kb on - strand, within BWI76_RS00855 at 178.145 kb on - strand at 178.161 kb on - strand at 178.161 kb on - strand at 178.175 kb on + strand at 178.551 kb on - strand, within BWI76_RS00865 at 178.618 kb on - strand, within BWI76_RS00865 at 178.761 kb on - strand, within BWI76_RS00865 at 178.943 kb on + strand, within BWI76_RS00865 at 179.153 kb on + strand, within BWI76_RS00865 at 179.208 kb on - strand, within BWI76_RS00865 at 179.279 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_noCarbon with Mixed culture; Ralstonia sp. UNC404CL21Col 3:1 starting OD to Klebsiella and Glu_DLac_Acetate_mix 0.1 x remove 177,458 + BWI76_RS00855 0.49 -1.3 177,466 - BWI76_RS00855 0.49 -0.6 177,598 + BWI76_RS00855 0.59 -0.5 177,712 + BWI76_RS00855 0.67 +1.0 177,719 - BWI76_RS00855 0.68 -2.7 177,744 - BWI76_RS00855 0.70 -3.2 178,145 - +0.6 178,161 - +0.4 178,161 - +1.0 178,175 + -0.2 178,551 - BWI76_RS00865 0.13 +1.6 178,618 - BWI76_RS00865 0.20 +1.8 178,761 - BWI76_RS00865 0.36 +1.0 178,943 + BWI76_RS00865 0.56 +1.0 179,153 + BWI76_RS00865 0.79 +1.8 179,208 - BWI76_RS00865 0.85 -2.3 179,279 - +2.6
Or see this region's nucleotide sequence